SPI1 antibody (Middle Region)

Catalog No. ABIN2780474

Product Details anti-SPI1 Antibody

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPI1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: QKGNRKKMTY QKMARALRNY GKTGEVKKVK KKLTYQFSGE VLGRGGLAER

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 85%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SPI1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SPI1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 270 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: SPI1 (SPI1 Products)

Synonyms: OF antibody, PU.1 antibody, SFPI1 antibody, SPI-1 antibody, SPI-A antibody, Dis-1 antibody, Dis1 antibody, Sfpi-1 antibody, Sfpi1 antibody, Spi-1 antibody, Tcfpu1 antibody, Tfpu.1 antibody, Pu.1 antibody, cb1086 antibody, pu.1 antibody, pu1 antibody, sfpi1 antibody, si:by184l24.2 antibody, spi1 antibody, SPI1/PU.1 antibody, spi1l antibody, zgc:152991 antibody, Spi-1 proto-oncogene antibody, spleen focus forming virus (SFFV) proviral integration oncogene antibody, Spi-1 proto-oncogene b antibody, Spi-1 proto-oncogene a antibody, SPI1 antibody, Spi1 antibody, spi1b antibody, spi1a antibody

Background: SPI1 is known to collaborate with multiple transcription factors and to regulate expression of immunoglobulin genes. Lack of SPI1 expression is associated with defective immunoglobulin transcription in Hodgkin and Reed-Sternberg cells of classical Hodgkin disease. This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: OF, PU.1, SFPI1, SPI-1, SPI-A
Protein Interaction Partner: HOXA10, TMX1, VAV1, IRF1, IRF8, GATA3, GATA2, GATA1, GFI1, TBP, RB1, BYRF1, RARA, PML, HDAC3, SKI, SIN3A, HDAC1, BCL6, CEBPA, PIP, SPIB, SSRP1, IRF4, KAT6A, CEBPE, NFATC1, CREBBP, NONO, CEBPB, JUN, ERG, NFKB1, MITF, FUS, CREM, ATF1, CEBPD, MECP2, DNMT3B,
Protein Size: 270

Molecular Weight: 31 kDa

Gene ID: 6688

NCBI Accession: NM_003120, NP_003111

UniProt: P17947

Pathways: Stem Cell Maintenance