MDS1 and EVI1 Complex Locus (MECOM) (N-Term) antibody

Details for Product No. ABIN2780631, Supplier: Log in to see
Antigen
  • evi1
  • im:7140716
  • prdm3
  • EVI1
  • AML1-EVI-1
  • MDS1
  • MDS1-EVI1
  • PRDM3
  • D630039M04Rik
  • Evi-1
  • Evi1
  • Jbo
  • Mds
  • Mds1
  • Mds1-Evi1
  • Prdm3
  • Znfpr1b1
  • AML1
  • AMLCR1
  • CBFA2
  • EVI-1
  • PEBP2aB
  • MDS1 and EVI1 complex locus
  • runt-related transcription factor 1
  • mecom
  • MECOM
  • LOC100343228
  • Mecom
  • RUNX1
Epitope
N-Term
28
17
17
15
9
5
5
4
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Zebrafish
105
33
9
4
4
3
3
2
1
1
Host
Rabbit
71
31
15
Clonality
Polyclonal
Conjugate
Un-conjugated
6
6
6
6
6
6
Application
Western Blotting (WB)
104
65
11
6
3
2
1
1
1
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human MDS1
Sequence MRSKGRARKL ATNNECVYGN YPEIPLEEMP DADGVASTPS LNIQEPCSPA
Predicted Reactivity Human: 100%, Mouse: 93%, Zebrafish: 77%
Characteristics This is a rabbit polyclonal antibody against MDS1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name MDS1 (MECOM Antibody Abstract)
Background MDS1 is located at 3q26 170-400 kb upstream (telomeric) of EVI1 in the chromosomal region in which some of the breakpoints 5' of EVI1 have been mapped. MDS1 has been identified as a single gene as well as a previously unreported exon(s) of EVI1. MDS1 exists in normal tissues both as a unique transcript and as a normal fusion transcript with EVI1, with an additional 188 codons at the 5' end of the previously reported EVI1 open reading frame. In cells with translocation t (3,21), additional fusion transcripts are AML1-MDS1 and AML1-MDS1-EVI1. EVI1 and MDS1 are involved in leukemia associated with chromosomal translocation breakpoints in the region between these genes.
Alias Symbols: PRDM3, MDS1-EVI1
Protein Size: 169
Molecular Weight 19 kDa
Gene ID 4197
NCBI Accession NM_004991, NP_004982
UniProt Q13465
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 169 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-MDS1 and EVI1 Complex Locus (MECOM) (N-Term) antibody (ABIN2780631) WB Suggested Anti-MDS1 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:...
Western Blotting (WB) image for anti-MDS1 and EVI1 Complex Locus (MECOM) (N-Term) antibody (ABIN2780631) WB Suggested Anti-MDS1
Antibody Titration: 1.25 µg/mL ELISA Titer: 1:312500
Background publications Fears, Mathieu, Zeleznik-Le, Huang, Rowley, Nucifora: "Intergenic splicing of MDS1 and EVI1 occurs in normal tissues as well as in myeloid leukemia and produces a new member of the PR domain family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 4, pp. 1642-7, 1996 (PubMed).

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