TSG101 antibody (C-Term)

Catalog No. ABIN2780762

Product Details anti-TSG101 Antibody

Target: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Goat, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSG101 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: TIFYLGEALR RGVIDLDVFL KHVRLLSRKQ FQLRALMQKA RKTAGLSDLY

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against TSG101. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TSG101

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 390 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TSG101 antibody (ABIN2780762)

Surjit, Oberoi, Kumar, Lal: "Enhanced alpha1 microglobulin secretion from Hepatitis E virus ORF3-expressing human hepatoma cells is mediated by the tumor susceptibility gene 101." in: The Journal of biological chemistry, Vol. 281, Issue 12, pp. 8135-42, (2006) (PubMed).

Target Details for TSG101

Target: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Alternative Name: TSG101 (TSG101 Products)

Background: TSG101 belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. TSG101 contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. TSG101 may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of TSG101 appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in TSG101 gene occur in high frequency in breast cancer. The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
Alias Symbols: TSG10, VPS23
Protein Interaction Partner: USHBP1, CEP55, VPS37C, VPS28, AATF, MGRN1, PDLIM7, TAX1BP1, KRT31, KRT13, KIFC3, DAPK3, AR, FAM9B, VPS37A, HAUS1, SYCE1, UBC, LRSAM1, ADRB2, MVB12A, UBAP1, CDKN1A, HGS, gag, gag-pol, GRB2, CDS2, ZFYVE9, VCP, VPS37B, WDR12, XPO1, UBD, ARRDC1, KCNN4, RAB11F
Protein Size: 390

Molecular Weight: 44 kDa

Gene ID: 7251

NCBI Accession: NM_006292, NP_006283

UniProt: Q99816