ZEB2 antibody (Middle Region)

Catalog No. ABIN2780948

Product Details anti-ZEB2 Antibody

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Horse, Guinea Pig, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZEB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: LGRQDGDEEF EEEEEESENK SMDTDPETIR DEEETGDHSM DDSSEDGKME

Predicted Reactivity: Cow: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ZEB2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ZEB2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1214 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ZEB2 antibody (ABIN2780948)

Yasumi, Inoue, Maruhashi, Kamachi, Higashi, Kondoh, Uchikawa: "Regulation of trunk neural crest delamination by δEF1 and Sip1 in the chicken embryo." in: Development, growth & differentiation, Vol. 58, Issue 2, pp. 205-14, (2016) (PubMed).

Guan, Liang, Xie, Li, Liu, Liu, Xiu, Li: "Down-regulation of miR-144 promotes thyroid cancer cell invasion by targeting ZEB1 and ZEB2." in: Endocrine, Vol. 48, Issue 2, pp. 566-74, (2015) (PubMed).

Rogers, Saxena, Bronner: "Sip1 mediates an E-cadherin-to-N-cadherin switch during cranial neural crest EMT." in: The Journal of cell biology, Vol. 203, Issue 5, pp. 835-47, (2013) (PubMed).

Target Details for ZEB2

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Alternative Name: ZEB2 (ZEB2 Products)

Background: The ZFHX1B gene is a member of the delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain proteins. ZFHX1B is strongly transcribed at an early stage in the developing peripheral and central nervous systems of both mice and humans, in all neuronal regions of the brains of 25-week human fetuses and adult mice, and in numerous nonneural tissues. The SMADIP1 gene (also known as SIP1) is a member of the delta-EF1 (ZEB1, MIM 189909)/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SMADIP1 interacts with receptor-mediated, activated full-length SMADs (see MIM 605568) (Verschueren et al., 1999 [PubMed 10400677]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-58 AB056507.1 1-58 59-553 AL118674.1 57-551 554-5558 AB056507.1 553-5557 5559-5583 AI858477.1 1-25 c
Alias Symbols: KIAA0569, SIP-1, SIP1, SMADIP1, ZFHX1B, HSPC082
Protein Interaction Partner: CTBP1, SOX2, SMAD9, SMAD3, SMAD2, SMAD1, APP, HDAC4, CBX4, SUMO1, UBE2I, ELAVL1, UBC, MTA2, MTA1, RBBP7, RBBP4, HDAC2, HDAC1, CEBPA, COPS6, SMAD5, CTBP2,
Protein Size: 1214

Molecular Weight: 136 kDa

Gene ID: 9839

NCBI Accession: NM_014795, NP_055610

UniProt: O60315

Pathways: Tube Formation