CHAF1B antibody (N-Term)

Catalog No. ABIN2782376

Product Details anti-CHAF1B Antibody

Target: CHAF1B (Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHAF1B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KVITCEIAWH NKEPVYSLDF QHGTAGRIHR LASAGVDTNV RIWKVEKGPD

Predicted Reactivity: Cow: 92%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against CHAF1B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CHAF1B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 559 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CHAF1B antibody (ABIN2782376)

Spellman, Ahmed, Dubach, Gardiner: "Expression of trisomic proteins in Down syndrome model systems." in: Gene, Vol. 512, Issue 2, pp. 219-25, (2012) (PubMed).

Target Details for CHAF1B

Target: CHAF1B (Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))

Alternative Name: CHAF1B (CHAF1B Products)

Synonyms: CHAF1B antibody, wu:fd07c09 antibody, wu:fe36g06 antibody, wu:fv38g09 antibody, zgc:56096 antibody, CAF-1 antibody, CAF-IP60 antibody, CAF1 antibody, CAF1A antibody, CAF1P60 antibody, MPHOSPH7 antibody, MPP7 antibody, 2600017H24Rik antibody, C76145 antibody, CAF-I p60 antibody, CAF-Ip60 antibody, caf-1 antibody, CAF-1P60 antibody, chromatin assembly factor 1 subunit B antibody, chromatin assembly factor 1, subunit B antibody, chromatin assembly factor 1, subunit B (p60) antibody, chromatin assembly factor 1 subunit B L homeolog antibody, CHAF1B antibody, chaf1b antibody, Chaf1b antibody, chaf1b.L antibody

Background: Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. CHAF1B corresponds to the p60 subunit and is required for chromatin assembly after replication. CHAF1B is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7
Protein Interaction Partner: UBC, SOX2, CNOT6L, env, CBX6, CBX5, HIST1H3B, HIST1H3A, CHAF1A, PCNA, APP, HIST2H3C, HIST1H4F, HIST1H3E, HIST3H3, SUMO2, BANF1, ASF1B, ASF1A, SETDB1, RBBP4, CDK2, CDK1,
Protein Size: 559

Molecular Weight: 61 kDa

Gene ID: 8208

NCBI Accession: NM_005441, NP_005432

UniProt: Q13112