PDK3 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 3) (Middle Region)

Details for Product anti-PDK3 Antibody No. ABIN2782500, Supplier: Log in to see
Antigen
  • pdk3
  • PDK3
  • 2610001M10Rik
  • AI035637
  • pyruvate dehydrogenase kinase, isozyme 3a
  • pyruvate dehydrogenase kinase, isozyme 3
  • pyruvate dehydrogenase kinase, isoenzyme 3
  • pdk3a
  • PDK3
  • pdk3
  • LOC100226102
  • Pdk3
Alternatives
anti-Human PDK3 antibody for Western Blotting
Epitope
Middle Region
16
12
12
10
8
5
3
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
64
32
7
3
3
3
2
2
2
2
2
2
1
1
Host
Rabbit
76
9
Clonality
Polyclonal
Conjugate
This PDK3 antibody is un-conjugated
6
6
6
6
6
6
Application
Western Blotting (WB)
79
52
23
6
4
4
3
3
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PDK3
Blocking Peptide Blocking peptide for this product available: ABIN981540
Sequence IYLKALSSES FERLPVFNKS AWRHYKTTPE ADDWSNPSSE PRDASKYKAK
Predicted Reactivity Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 85%
Characteristics This is a rabbit polyclonal antibody against PDK3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name PDK3 (PDK3 Antibody Abstract)
Background PDK3 belongs to the PDK/BCKDK protein kinase family. It contains 1 histidine kinase domain. PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Alias Symbols: -
Protein Interaction Partner: APP, UBD, UBC, ICT1, EGFR, DLAT, PDHA1,
Protein Size: 406
Molecular Weight 47 kDa
Gene ID 5165
NCBI Accession NM_005391, NP_005382
UniProt Q15120
Research Area Signaling, Metabolism
Pathways PI3K-Akt Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 406 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) (Middle Region) antibody (ABIN2782500) WB Suggested Anti-PDK3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Background publications Degenhardt, Saramäki, Malinen, Rieck, Väisänen, Huotari, Herzig, Müller, Carlberg: "Three members of the human pyruvate dehydrogenase kinase gene family are direct targets of the peroxisome proliferator-activated receptor beta/delta." in: Journal of molecular biology, Vol. 372, Issue 2, pp. 341-55, 2007 (PubMed).

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