MTHFD1 antibody (Middle Region)
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- Target See all MTHFD1 Antibodies
- MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))
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Binding Specificity
- Middle Region
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Reactivity
- Human, Mouse, Rat, Rabbit, Horse, Zebrafish (Danio rerio), Pig, Dog, Guinea Pig, Cow, Saccharomyces cerevisiae
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MTHFD1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Sequence
- CMAKTHLSLS HNPEQKGVPT GFILPIRDIR ASVGAGFLYP LVGTMSTMPG
- Predicted Reactivity
- Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Yeast: 79%, Zebrafish: 79%
- Characteristics
- This is a rabbit polyclonal antibody against MTHFD1. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the middle region of human MTHFD1
- Top Product
- Discover our top product MTHFD1 Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 935 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))
- Alternative Name
- MTHFD1 (MTHFD1 Products)
- Background
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MTHFD1 possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MTHFC, MTHFD
Protein Interaction Partner: UBC, FUS, SUMO2, SUMO3, NEDD8, MDM2, RPA3, RPA2, RPA1, RNF2, RPS21, MAT2A, UBQLN1, FBXO6, UBD, ADRB2, CD81, VCAM1, HSP90AA1, HSP90AB1, PRKCDBP, SFXN1, TMEM165, IARS2, SARS2, SF3B3, FAF2, PPT2, SNRNP70, RPL32, MRPL23, ALDH18A1, PRPH, PC, ICT1, GAA, ACSL3,
Protein Size: 935 - Molecular Weight
- 101 kDa
- Gene ID
- 4522
- NCBI Accession
- NM_005956, NP_005947
- UniProt
- P11586
- Pathways
- Methionine Biosynthetic Process
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