EIF3E antibody (Middle Region)

Catalog No. ABIN2783260

Product Details anti-EIF3E Antibody

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: LNMTPEEAER WIVNLIRNAR LDAKIDSKLG HVVMGNNAVS PYQQVIEKTK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF3E. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EIF3E

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 445 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF3E

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Alternative Name: EIF3E (EIF3E Products)

Synonyms: EIF3-P48 antibody, EIF3S6 antibody, INT6 antibody, eIF3-p46 antibody, 48kDa antibody, Eif3s6 antibody, Int6 antibody, eIF3-p48 antibody, eif3-p48 antibody, eif3s6 antibody, eif3s6-A antibody, eIF3e antibody, eIF3-S6 antibody, eif3s6-B antibody, EIF3SE antibody, eif3s6b antibody, im:7147439 antibody, QtsA-18056 antibody, eukaryotic translation initiation factor 3 subunit E antibody, eukaryotic translation initiation factor 3, subunit E antibody, eukaryotic translation initiation factor 3 subunit E L homeolog antibody, eukaryotic translation initiation factor 3 subunit 6 antibody, eukaryotic translation initiation factor 3 subunit E S homeolog antibody, eukaryotic translation initiation factor 3, subunit E, a antibody, Eukaryotic translation initiation factor 3 subunit E antibody, eukaryotic translation initiation factor 3, subunit E, b antibody, EIF3E antibody, Eif3e antibody, eif3e antibody, eif3e.L antibody, LOC692936 antibody, eif3e.S antibody, eif3ea antibody, eif-3.E antibody, eif3eb antibody, LOC108348260 antibody, int6 antibody

Background: EIF3E belongs to the eIF-3 subunit E family.It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. EIF3E is required for nonsense-mediated mRNA decay (NMD), It may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. The protein may interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
Alias Symbols: EIF3-P48, EIF3S6, INT6, eIF3-p46
Protein Interaction Partner: UBC, C4orf19, RUNDC3A, HUWE1, TRIM63, TRIM55, STAU1, SUMO1, NEDD8, GPRASP2, LARP1, GNB2L1, RPS28, RPS25, RPS23, RPS20, RPS19, RPS18, RPS12, RPS10, RPS9, RPS8, RPS3A, RPS3, RPS2, EIF3CL, DYNC1LI1, FBXO6, EIF3D, IGSF8, NPM1, ICAM1, CD81, EIF3B, VCAM1, POLR2
Protein Size: 445

Molecular Weight: 52 kDa

Gene ID: 3646

NCBI Accession: NM_001568, NP_001559

UniProt: P60228

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C