GADD45B antibody (Middle Region)

Catalog No. ABIN2783334

Product Details anti-GADD45B Antibody

Target: GADD45B (Growth Arrest and DNA-Damage-Inducible, beta (GADD45B))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GADD45B antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Sequence: FCCDNDINIV RVSGMQRLAQ LLGEPAETQG TTEARDLHCL LVTNPHTDAW

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against GADD45B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GADD45B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 160 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GADD45B antibody (ABIN2783334)

Ursini, Cavalleri, Fazio, Angrisano, Iacovelli, Porcelli, Maddalena, Punzi, Mancini, Gelao, Romano, Masellis, Calabrese, Rampino, Taurisano, Di Giorgio, Keller, Tarantini, Sinibaldi, Quarto et al.: "BDNF rs6265 methylation and genotype interact on risk for schizophrenia. ..." in: Epigenetics, Vol. 11, Issue 1, pp. 11-23, (2016) (PubMed).

Gavin, Kusumo, Zhang, Guidotti, Pandey: "Role of Growth Arrest and DNA Damage-Inducible, Beta in Alcohol-Drinking Behaviors." in: Alcoholism, clinical and experimental research, Vol. 40, Issue 2, pp. 263-72, (2016) (PubMed).

Matrisciano, Dong, Gavin, Nicoletti, Guidotti: "Activation of group II metabotropic glutamate receptors promotes DNA demethylation in the mouse brain." in: Molecular pharmacology, Vol. 80, Issue 1, pp. 174-82, (2011) (PubMed).

Gavin, Sharma, Chase, Matrisciano, Dong, Guidotti: "Growth arrest and DNA-damage-inducible, beta (GADD45b)-mediated DNA demethylation in major psychosis." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 37, Issue 2, pp. 531-42, (2011) (PubMed).

Tornatore, Marasco, Dathan, Vitale, Benedetti, Papa, Franzoso, Ruvo, Monti: "Gadd45 beta forms a homodimeric complex that binds tightly to MKK7." in: Journal of molecular biology, Vol. 378, Issue 1, pp. 97-111, (2008) (PubMed).

Target Details for GADD45B

Target: GADD45B (Growth Arrest and DNA-Damage-Inducible, beta (GADD45B))

Alternative Name: GADD45B (GADD45B Products)

Synonyms: GADD45BETA antibody, MYD118 antibody, AI323528 antibody, Myd118 antibody, CS131 antibody, cb196 antibody, gadd45b antibody, gadd45b2 antibody, sb:cb196 antibody, zgc:73104 antibody, gadd45beta antibody, wu:fa92d10 antibody, wu:fa98h12 antibody, wu:fk65h03 antibody, zgc:111973 antibody, gadd45b1 antibody, gadd45bl antibody, zgc:112453 antibody, growth arrest and DNA damage inducible beta antibody, growth arrest and DNA-damage-inducible 45 beta antibody, growth arrest and DNA-damage-inducible, beta antibody, growth arrest and DNA-damage-inducible, beta a antibody, growth arrest and DNA-damage-inducible, beta b antibody, GADD45B antibody, Gadd45b antibody, gadd45ba antibody, gadd45bb antibody

Background: The function of GADD45B is involved in the regulation of growth and apoptosis.This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The genes in this group respond to environmental stresses by mediating activation of the p38/JNK pathway. This activation is mediated via their proteins binding and activating MTK1/MEKK4 kinase, which is an upstream activator of both p38 and JNK MAPKs. The function of these genes or their protein products is involved in the regulation of growth and apoptosis. These genes are regulated by different mechanisms, but they are often coordinately expressed and can function cooperatively in inhibiting cell growth. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DKFZP566B133, GADD45BETA, MYD118
Protein Interaction Partner: UBD, UBC, MAP3K4, GADD45GIP1, GADD45G, MAP2K7, MAP3K5, PPARG, PPARD, PCNA, PPARA, ESR1, CCNB1, CDK1, CDKN1A, GADD45A, EGR1,
Protein Size: 160

Molecular Weight: 18 kDa

Gene ID: 4616

NCBI Accession: NM_015675, NP_056490

UniProt: O75293

Pathways: Cell Division Cycle