MPST antibody (Middle Region)

Catalog No. ABIN2783349

Product Details anti-MPST Antibody

Target: MPST (Mercaptopyruvate Sulfurtransferase (MPST))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Cow, Dog, Horse, Goat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPST antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DPAFIKTYED IKENLESRRF QVVDSRATGR FRGTEPEPRD GIEPGHIPGT

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against MPST. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MPST

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 297 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MPST antibody (ABIN2783349)

Cui, Navneet, Wang, Roon, Chen, Xian, Smith: "Analysis of MTHFR, CBS, Glutathione, Taurine, and Hydrogen Sulfide Levels in Retinas of Hyperhomocysteinemic Mice." in: Investigative ophthalmology & visual science, Vol. 58, Issue 4, pp. 1954-1963, (2017) (PubMed).

Otani, Nagaoka, Omae, Tanano, Kamiya, Ono, Hein, Kuo, Yoshida: "Histamine-Induced Dilation of Isolated Porcine Retinal Arterioles: Role of Endothelium-Derived Hyperpolarizing Factor." in: Investigative ophthalmology & visual science, Vol. 57, Issue 11, pp. 4791-8, (2016) (PubMed).

Lo, Tsai, Tsai, Hua, Tsai, Huang, Tsai, Lai: "Identification of over-expressed proteins in oral squamous cell carcinoma (OSCC) patients by clinical proteomic analysis." in: Clinica chimica acta; international journal of clinical chemistry, Vol. 376, Issue 1-2, pp. 101-7, (2006) (PubMed).

Target Details for MPST

Target: MPST (Mercaptopyruvate Sulfurtransferase (MPST))

Alternative Name: MPST (MPST Products)

Background: MPST transfer of a sulfur ion to cyanide or to other thiol compounds. MPST also has weak rhodanese activity. MPST may have a role in cyanide degradation or in thiosulfate biosynthesis.This gene encodes a protein which can function as a monomer or as a disulfide-linked homodimer and which catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cyanide degradation and in thiosulfate biosynthesis. The encoded cytoplasmic protein is a member of the rhodanese family but is not rhodanese itself, which is a mitochondrial protein. Alternatively spliced transcript variants encoding the same protein have been identified.
Alias Symbols: MGC24539, MST, TST2
Protein Interaction Partner: UBC, NEDD8, TMEM189, S100A16, NADK2, SEPT11, TIMM9, YKT6, SEC22B, VAPB, UQCRFS1, UPP1, TPI1, NDUFV1, ANXA4, RAE1,
Protein Size: 297

Molecular Weight: 33 kDa

Gene ID: 4357

NCBI Accession: NM_021126, NP_066949

UniProt: P25325