Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

POLR3A antibody (Middle Region)

POLR3A Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Horse, Guinea Pig, Saccharomyces cerevisiae WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2783481
  • Target See all POLR3A Antibodies
    POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
    Binding Specificity
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Middle Region
    Reactivity
    • 26
    • 15
    • 10
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Rabbit, Dog, Cow, Horse, Guinea Pig, Saccharomyces cerevisiae
    Host
    • 26
    Rabbit
    Clonality
    • 26
    Polyclonal
    Conjugate
    • 15
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This POLR3A antibody is un-conjugated
    Application
    • 14
    • 10
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Sequence
    AYFGQKDSVC GVSECIIMGI PMNIGTGLFK LLHKADRDPN PPKRPLIFDT
    Predicted Reactivity
    Cow: 79%, Dog: 86%, Guinea Pig: 86%, Horse: 79%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 86%, Yeast: 85%
    Characteristics
    This is a rabbit polyclonal antibody against POLR3A. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the middle region of human POLR3A
    Top Product
    Discover our top product POLR3A Primary Antibody
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 1390 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
    Alternative Name
    POLR3A (POLR3A Products)
    Synonyms
    9330175N20Rik antibody, BC053071 antibody, RPC1 antibody, RPC155 antibody, RGD1305574 antibody, ADDH antibody, HLD7 antibody, hRPC155 antibody, polymerase (RNA) III (DNA directed) polypeptide A antibody, RNA polymerase III subunit A antibody, Polr3a antibody, POLR3A antibody
    Background
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
    Alias Symbols: RPC1, RPC155, hRPC155, ADDH, HLD7
    Protein Interaction Partner: SUMO2, UBC, ASB13, NEDD4, POLR3H, VPS18, POLR3B, POLR1D, CRCP, POLR1A, POLR3C, POLR1C, SMARCA5, POLR2H, POLR2E, POLR3D, IKBKG, PCBD1, EWSR1, RPAP2, RPAP3, RPAP1, GPN1, RAE1, POLR3E, PKP2, GTF2B,
    Protein Size: 1390
    Molecular Weight
    156 kDa
    Gene ID
    11128
    NCBI Accession
    NM_007055, NP_008986
    UniProt
    O14802
You are here:
Support