POLR1B antibody (Middle Region)

Catalog No. ABIN2783529

Product Details anti-POLR1B Antibody

Target: POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SDKFQVRTTG ARDRVTNQPI GGRNVQGGIR FGEMERDALL AHGTSFLLHD

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 100%, Rat: 93%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against POLR1B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POLR1B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1135 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLR1B

Target: POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))

Alternative Name: POLR1B (POLR1B Products)

Synonyms: RPOL-1l antibody, MGC68946 antibody, 128kDa antibody, D630020H17Rik antibody, RPA116 antibody, RPA135 antibody, RPA2 antibody, Rpo1-2 antibody, RNA polymerase I subunit B antibody, polymerase (RNA) I polypeptide B L homeolog antibody, polymerase (RNA) I polypeptide B, 128kDa antibody, polymerase (RNA) I polypeptide B antibody, DNA-directed RNA polymerase I subunit RPA2 antibody, POLR1B antibody, polr1b.L antibody, polr1b antibody, LOC100563266 antibody, LOC100634236 antibody, Polr1b antibody

Background: Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).
Alias Symbols: FLJ10816, FLJ21921, MGC131780, RPA135, Rpo1-2, RPA2
Protein Interaction Partner: UBC, RPA3, RPA2, RPA1, RRN3, HDAC6, GIT2, MED20, NMI, LDB1, ZNF207, RPL37, CCND1, AKT2, ACTA1, ERBB2, ACTB, TSR1, POLR1D, POLR1A, CD3EAP, NOP56, POLR1C, DHX8, CAND1, ARRB2, LZTR1, TAF1D, USP11, POLR1E, EIF3L, NFKBIB, POLR1B, ESR1, RIBIN,
Protein Size: 1135

Molecular Weight: 128 kDa

Gene ID: 84172

NCBI Accession: NM_019014, NP_061887

UniProt: Q9H9Y6