MANEA antibody (Middle Region)

Catalog No. ABIN2783932

Product Details anti-MANEA Antibody

Target: MANEA (Mannosidase, Endo-alpha (MANEA))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Horse, Zebrafish (Danio rerio), Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MANEA antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KVTFHIEPYS NRDDQNMYKN VKYIIDKYGN HPAFYRYKTK TGNALPMFYV

Predicted Reactivity: Dog: 100%, Horse: 86%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 92%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against MANEA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MANEA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 462 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MANEA

Target: MANEA (Mannosidase, Endo-alpha (MANEA))

Alternative Name: MANEA (MANEA Products)

Synonyms: ENDO antibody, hEndo antibody, Enman antibody, 4932703L02Rik antibody, fi29h09 antibody, zgc:92825 antibody, wu:fi29h09 antibody, mannosidase endo-alpha antibody, mannosidase, endo-alpha antibody, MANEA antibody, Manea antibody, manea antibody

Background: N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans.
Alias Symbols: DKFZp686D20120, FLJ12838, hEndo, ENDO
Protein Interaction Partner: UBC, DNM2, PRD, AMPH,
Protein Size: 462

Molecular Weight: 54 kDa

Gene ID: 79694

NCBI Accession: NM_024641, NP_078917

UniProt: Q5SRI9