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SIGLEC12 antibody (N-Term)

SIGLEC12 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2784108
  • Target See all SIGLEC12 Antibodies
    SIGLEC12 (Sialic Acid Binding Ig-Like Lectin 12 (SIGLEC12))
    Binding Specificity
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 28
    • 15
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 29
    • 3
    • 1
    Rabbit
    Clonality
    • 31
    • 2
    Polyclonal
    Conjugate
    • 21
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SIGLEC12 antibody is un-conjugated
    Application
    • 25
    • 11
    • 8
    • 3
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB)
    Sequence
    DTRESDAGTY VFCVERGNMK WNYKYDQLSV NVTASQDLLS RYRLEVPESV
    Predicted Reactivity
    Human: 100%
    Characteristics
    This is a rabbit polyclonal antibody against SIGLEC12. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the N terminal region of human SIGLEC12
    Top Product
    Discover our top product SIGLEC12 Primary Antibody
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 595 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    SIGLEC12 (Sialic Acid Binding Ig-Like Lectin 12 (SIGLEC12))
    Alternative Name
    SIGLEC12 (SIGLEC12 Products)
    Synonyms
    S2V antibody, SIGLECL1 antibody, SLG antibody, Siglec-XII antibody, sialic acid binding Ig like lectin 12 (gene/pseudogene) antibody, sialic acid binding Ig like lectin 12 antibody, sialic acid binding Ig-like lectin 12 antibody, SIGLEC12 antibody
    Background
    Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. SIGLEC12 is a member of the SIGLEC3-like subfamily of SIGLECs. SIGLEC12, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor.Western blots using four different antibodies against four unique regions of this protein target confirm the same apparent molecular weight in our tests.Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene.
    Alias Symbols: FLJ38600, S2V, SIGLECL1, SLG, Siglec-12, Siglec-L1, Siglec-XII
    Protein Interaction Partner: CMAS, PTPN6, PTPN11,
    Protein Size: 595
    Molecular Weight
    63 kDa
    Gene ID
    89858
    NCBI Accession
    NM_053003, NP_443729
    UniProt
    Q96PQ1
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