BCKDK antibody (N-Term)

Catalog No. ABIN2784788

Product Details anti-BCKDK Antibody

Target: BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCKDK antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: CLPFIIGCNP TILHVHELYI RAFQKLTDFP PIKDQADEAQ YCQLVRQLLD

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 92%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against BCKDK. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BCKDK

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 412 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-BCKDK antibody (ABIN2784788)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for BCKDK

Target: BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))

Alternative Name: BCKDK (BCKDK Products)

Synonyms: cb233 antibody, zgc:55984 antibody, wu:fa04g07 antibody, wu:fb80b08 antibody, MGC78818 antibody, BCKDK antibody, AI327402 antibody, BCKDKD antibody, BDK antibody, branched chain ketoacid dehydrogenase kinase antibody, branched chain ketoacid dehydrogenase kinase S homeolog antibody, bckdk antibody, bckdk.S antibody, BCKDK antibody, Bckdk antibody

Background: BCKDK belongs to the PDK/BCKDK protein kinase family. It contains 1 histidine kinase domain. BCKDK catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. BCKDK is the key enzyme that regulates the activity state of the BCKD complex.
Alias Symbols: -
Protein Interaction Partner: UBC, SUMO1, NEDD8, LSM8, STAT3, EPS8, APP, NR5A2, TERF2, TRAF4, FAM98B, CAB39L, RNF219, CAB39, RTCA, BCKDHA,
Protein Size: 412

Molecular Weight: 43 kDa

Gene ID: 10295

NCBI Accession: NM_005881, NP_005872

UniProt: O14874

Pathways: SARS-CoV-2 Protein Interactome