MED8 antibody (N-Term)

Catalog No. ABIN2784933

Product Details anti-MED8 Antibody

Target: MED8 (Mediator Complex Subunit 8 (MED8))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED8 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MQREEKQLEA SLDALLSQVA DLKNSLGSFI CKLENEYGRL TWPSVLDSFA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against MED8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MED8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 301 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED8

Target: MED8 (Mediator Complex Subunit 8 (MED8))

Alternative Name: MED8 (MED8 Products)

Synonyms: ARC32 antibody, 2210021A15Rik antibody, AB041805 antibody, MNCb-2386 antibody, zgc:76877 antibody, med8-a antibody, mediator complex subunit 8 antibody, mediator complex subunit 8 L homeolog antibody, component of RNA polymerase II antibody, mediator complex subunit Med8 antibody, MED8 antibody, Med8 antibody, med8 antibody, med8.L antibody

Background: MED8 is a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. MED8 also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase.This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.
Alias Symbols: ARC32, MGC17544, MGC19641
Protein Interaction Partner: PNMA1, KRT40, CDK19, CDK8, MED19, MED26, FBXW7, EPAS1, MED29, MED18, CPSF1, MED4, MED16, MED12, MED24, MED21, MED14, MED1, HIST1H1E, APP, MED11, SUMO2, MED10, RBX1, CTDP1, CUL2, TCEB2, TCEB1, MED28, OBFC1, ZC3H13, TRIP4, TADA2A, THOC7, PHYHIP, MED20, MED9
Protein Size: 301

Molecular Weight: 33 kDa

Gene ID: 112950

NCBI Accession: NM_052877, NP_443109

Pathways: Regulation of Lipid Metabolism by PPARalpha