PIAS2 antibody (N-Term)

Catalog No. ABIN2785610

Product Details anti-PIAS2 Antibody

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RELYRRRYPR TLEGLSDLST IKSSVFSLDG GSSPVEPDLA VAGIHSLPST

Predicted Reactivity: Cow: 93%, Dog: 92%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 92%, Pig: 93%, Rabbit: 92%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against PIAS2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PIAS2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 572 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PIAS2

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Alternative Name: PIAS2 (PIAS2 Products)

Synonyms: MGC83751 antibody, piasx antibody, wu:fi05f09 antibody, PIAS2 antibody, 6330408K17Rik antibody, AI462206 antibody, ARIP3 antibody, AU018068 antibody, Dib antibody, Miz1 antibody, PIASxalpha antibody, PIASxb antibody, PIASxbeta antibody, SIZ2 antibody, DIP antibody, MIZ antibody, MIZ1 antibody, PIASX antibody, PIASX-ALPHA antibody, PIASX-BETA antibody, ZMIZ4 antibody, protein inhibitor of activated STAT 2 L homeolog antibody, protein inhibitor of activated STAT 2 antibody, protein inhibitor of activated STAT, 2 antibody, pias2.L antibody, PIAS2 antibody, pias2 antibody, Pias2 antibody

Background: Pias2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. It plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It seems to be mostly involved in gene silencing. It binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described.
Alias Symbols: MGC102682, MIZ1, PIASX-ALPHA, PIASX-BETA, SIZ2, ZMIZ4, miz, DIP, MIZ, ARIP3, PIASX
Protein Interaction Partner: HIC1, GOLGA2, GFAP, PHC1, TRIM23, ZBTB8A, SUMO1P1, ACY3, TSR2, NAV2, CCHCR1, CCNDBP1, MAPKAPK2, ZBED1, PIAS2, SUMO1, UBE2I, TP53, PRKAB2, MX2, TRIM63, TRIM55, CTBP1, GTF2I, SUMO2, SUMO3, MYC, DNMT3A, TICAM2, RUFY1, PIAS4, C1QA, ADA, PAXIP1, UBC, PLAGL1, P
Protein Size: 572

Molecular Weight: 63 kDa

Gene ID: 9063

NCBI Accession: NM_173206, NP_775298

Pathways: JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling