ACAT1 antibody (Middle Region)

Catalog No. ABIN2785629

Product Details anti-ACAT1 Antibody

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: SYTRSKAAWE AGKFGNEVIP VTVTVKGQPD VVVKEDEEYK RVDFSKVPKL

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against ACAT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ACAT1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 427 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ACAT1

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Alternative Name: ACAT1 (ACAT1 Products)

Synonyms: ACAT antibody, MAT antibody, T2 antibody, THIL antibody, RATACAL antibody, 6330585C21Rik antibody, Acat antibody, fd16h07 antibody, fd20g06 antibody, wu:fd16h07 antibody, wu:fd20g06 antibody, zgc:86832 antibody, acat1-a antibody, acetyl-CoA acetyltransferase 1 antibody, acetyl-Coenzyme A acetyltransferase 1 antibody, acetyl-CoA acetyltransferase 1 L homeolog antibody, ACAT1 antibody, Acat1 antibody, acat1 antibody, acat1.L antibody

Background: ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. The gene encoding ACAT1 spans approximately 27 kb and contains 12 exons interrupted by 11 introns. Defects in this gene are associated with the alpha-methylacetoaceticaciduria disorder, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. This gene spans approximately 27 kb and contains 12 exons interrupted by 11 introns. Defects in this gene are associated with the alpha-methylacetoaceticaciduria disorder, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: ACAT, MAT, T2, THIL
Protein Interaction Partner: SUMO2, MDM2, ACAT1, VCAM1, ITGA4, AGXT, FLOT1, UBAP2L, TTF2, UBL4A, UQCRFS1P1, UQCRB, SSBP1, SDHB, SDHA, SAFB, MARS, FOXM1, CSE1L, ACO2, TMEM65, TUBB1, TOMM7, TOPBP1, GCN1L1, TIMM44, ACAA2, FBXO6, PEX7, COPS5, CUL1, CUL3, SIRT7, SUMO1, RAD21, UBC, HDAC5,
Protein Size: 427

Molecular Weight: 41 kDa

Gene ID: 38

NCBI Accession: NM_000019, NP_000010

UniProt: P35610