FAM175B antibody (Middle Region)

Catalog No. ABIN2786185

Product Details anti-FAM175B Antibody

Target: FAM175B (Family with Sequence Similarity 175, Member B (FAM175B))

Binding Specificity: Middle Region

Reactivity: Mouse, Human, Dog, Rat, Rabbit, Cow, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAM175B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: FAAEGRSTLG DAEASDPPPP YSDFHPNNQE STLSHSRMER SVFMPRPQAV

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against KIAA0157. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human KIAA0157

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 415 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FAM175B antibody (ABIN2786185)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for FAM175B

Target: FAM175B (Family with Sequence Similarity 175, Member B (FAM175B))

Alternative Name: KIAA0157 (FAM175B Products)

Synonyms: RGD1308918 antibody, abro1 antibody, MGC83343 antibody, ABRO1 antibody, KIAA0157 antibody, AA589499 antibody, AI853413 antibody, Abro1 antibody, C430003P19Rik antibody, abraxas 2, BRISC complex subunit antibody, family with sequence similarity 175 member B antibody, abraxas 2, BRISC complex subunit L homeolog antibody, BRISC complex subunit antibody, Abraxas2 antibody, abraxas2 antibody, FAM175B antibody, abraxas2.L antibody, ABRAXAS2 antibody

Background: KIAA0157 is the component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. It may act as a central scaffold protein that assembles the various components of the BRISC complex.
Alias Symbols: ABRO1, FLJ22338, KIAA0157
Protein Interaction Partner: BRCC3, BABAM1, BRE, USP7, TP53, UBC, TXNDC9, HUWE1, SPAG9, HNRNPA2B1, ANKRD28, SMURF2, GRB2, ELAVL1, THAP5, UIMC1,
Protein Size: 415

Molecular Weight: 47 kDa

Gene ID: 23172

NCBI Accession: NM_032182, NP_115558

UniProt: Q15018