SKA1 antibody (Middle Region)

Catalog No. ABIN2786219

Product Details anti-SKA1 Antibody

Target: SKA1 (Spindle and Kinetochore Associated Complex Subunit 1 (SKA1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Horse, Pig, Rabbit, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKA1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LLNKFELEIQ YQEQTNSSLK ELCESLREEC EDVEHLKEHV PPHLPQVTAT

Predicted Reactivity: Cow: 93%, Dog: 93%, Horse: 86%, Human: 100%, Mouse: 93%, Pig: 79%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against Ska1. It was validated on Western Blot.

Purification: Affinity Purified

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 254 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SKA1

Target: SKA1 (Spindle and Kinetochore Associated Complex Subunit 1 (SKA1))

Alternative Name: Ska1 (SKA1 Products)

Synonyms: C18H18orf24 antibody, C18orf24 antibody, 2810433K01Rik antibody, AV117428 antibody, RGD1310784 antibody, spindle and kinetochore associated complex subunit 1 antibody, SKA1 antibody, ska1 antibody, Ska1 antibody

Background: Ska1 is a Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. It is required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules.
Alias Symbols: 2810433K01Rik, AV117428
Protein Size: 254

Molecular Weight: 28 kDa

Gene ID: 66468

NCBI Accession: NM_025581, NP_079857

UniProt: Q9CPV1

Pathways: M Phase