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PRAME antibody (N-Term)

PRAME Reactivity: Human, Mouse, Rat, Cow, Horse, Pig WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2786465
  • Target See all PRAME Antibodies
    PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
    Binding Specificity
    • 16
    • 9
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 43
    • 19
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Human, Mouse, Rat, Cow, Horse, Pig
    Host
    • 41
    • 2
    Rabbit
    Clonality
    • 43
    Polyclonal
    Conjugate
    • 22
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PRAME antibody is un-conjugated
    Application
    • 21
    • 16
    • 13
    • 13
    • 9
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB)
    Sequence
    MERRRLWGSI QSRYISMSVW TSPRRLVELA GQSLLKDEAL AIAALELLPR
    Predicted Reactivity
    Cow: 86%, Horse: 93%, Human: 100%, Mouse: 79%, Pig: 86%, Rat: 85%
    Characteristics
    This is a rabbit polyclonal antibody against PRAME. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the N terminal region of human PRAME
    Top Product
    Discover our top product PRAME Primary Antibody
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 509 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Liu, Zhao, Lu, Ning, Ma, Diaz, OConnor: "A novel testis-specific protein, PRAMEY, is involved in spermatogenesis in cattle." in: Reproduction (Cambridge, England), Vol. 153, Issue 6, pp. 847-863, (2017) (PubMed).

  • Target
    PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
    Alternative Name
    PRAME (PRAME Products)
    Synonyms
    PRAME antibody, CT130 antibody, MAPE antibody, OIP-4 antibody, OIP4 antibody, 4930534P07Rik antibody, preferentially expressed antigen in melanoma antibody, PRAME antibody, Prame antibody
    Background
    PRAME functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. PRAME prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene.
    Alias Symbols: MAPE, OIP4, CT130
    Protein Interaction Partner: PRAME, RBX1, CUL2, TCEB2, TCEB1, ZNF408, FBXL12, XRN2, HIST4H4, HIST2H2BE, HIST2H2AC, HIST3H3, UBC, RARA, TP53RK, OSGEP, TPRKB, KDM1A, BAG2, LAGE3, UBL4A, SNRPB, RPS13, RPL22, RPL10, RFC4, PSMC4, HNRNPM, HNRNPH1, HNRNPF, HNRNPC, NEDD8, STK19,
    Protein Size: 509
    Molecular Weight
    56 kDa
    Gene ID
    23532
    NCBI Accession
    NM_006115, NP_006106
    UniProt
    P78395
    Pathways
    Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding
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