AKTIP antibody (Middle Region)

Catalog No. ABIN2786570

Product Details anti-AKTIP Antibody

Target: AKTIP (AKT Interacting Protein (AKTIP))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKTIP antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: NPSVHDEARE KMLTQKKPEE QHNKSVHVAG LSWVKPGSVQ PFSKEEKTVA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 83%

Characteristics: This is a rabbit polyclonal antibody against AKTIP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human AKTIP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 292 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-AKTIP antibody (ABIN2786570)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for AKTIP

Target: AKTIP (AKT Interacting Protein (AKTIP))

Alternative Name: AKTIP (AKTIP Products)

Synonyms: FT1 antibody, FTS antibody, cb798 antibody, fts antibody, zgc:77699 antibody, fts-A antibody, aktip antibody, fts-B antibody, MGC133931 antibody, AL023020 antibody, Fif antibody, Ft antibody, Ft1 antibody, Fts antibody, AKT interacting protein antibody, akt interacting protein antibody, AKT interacting protein L homeolog antibody, AKT interacting protein S homeolog antibody, thymoma viral proto-oncogene 1 interacting protein antibody, AKTIP antibody, aktip antibody, aktip.L antibody, aktip.S antibody, Aktip antibody

Background: AKTIP is the component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). AKTIP regulates apoptosis by enhancing phosphorylation and activation of AKT1. AKTIP increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade.The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein.
Alias Symbols: FT1, FTS
Protein Interaction Partner: HOOK1, HOOK2, HOOK3, FAM160A2, VPS16, VPS18, VPS41, TRIM23, TRIM41, MARCH5, DZIP3, KIAA1377, EXOC7, POLA2, GTF3C1, UTP14A, C10orf2, PDPK1, ASS1, CTBP2, IMMT, RPA1, AKT1, TIMM50, PDS5A, IARS, HDLBP, DCTN1,
Protein Size: 292

Molecular Weight: 33 kDa

Gene ID: 64400

NCBI Accession: NM_001012398, NP_001012398

UniProt: Q9H8T0