HIST2H2BF antibody (Histone H2B Type 2-F) (N-Term)

Details for Product anti-HIST2H2BF Antibody No. ABIN2786582, Supplier: Log in to see
Antigen
  • histone cluster 2, H2bf
  • HIST2H2BF
Epitope
N-Term
12
10
8
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
20
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
20
Clonality
Polyclonal
Conjugate
This HIST2H2BF antibody is un-conjugated
2
2
2
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
19
14
Options
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human HIST2H2BF
Blocking Peptide Blocking peptide for this product available: ABIN978010
Sequence MPDPAKSAPA PKKGSKKAVT KVQKKDGKKR KRSRKESYSV YVYKVLKQVH
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against HIST2H2BF. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name HIST2H2BF (HIST2H2BF Antibody Abstract)
Background HIST2H2BF is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Alias Symbols: MGC131639
Protein Interaction Partner: SPRTN, UBC, rev, IGSF8, ICAM1, CD81, ITGA4,
Protein Size: 126
Molecular Weight 14 kDa
Gene ID 440689
NCBI Accession NM_001024599, NP_001019770
UniProt Q5QNW6
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 126 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-HIST2H2BF antibody (Histone H2B Type 2-F) (N-Term) (ABIN2786582) WB Suggested Anti-HIST2H2BF Antibody Titration: 0.2-1 ug/ml
ELISA Titer:...
Chromatin Immunoprecipitation (ChIP) image for anti-HIST2H2BF antibody (Histone H2B Type 2-F) (N-Term) (ABIN2786582) Chromatin Immunoprecipitation (ChIP) Using HIST2H2BF Antibody - N-terminal region (AR...
Background publications Kim, Sprung, Chen, Xu, Ball, Pei, Cheng, Kho, Xiao, Xiao, Grishin, White, Yang, Zhao: "Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." in: Molecular cell, Vol. 23, Issue 4, pp. 607-18, 2006 (PubMed).