Nemo-Like Kinase antibody (Middle Region)

Catalog No. ABIN2786944

Product Details anti-Nemo-Like Kinase Antibody

Target: Nemo-Like Kinase (NLK)

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nemo-Like Kinase antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RLRYHTCMCK CCFSTSTGRV YTSDFEPVTN PKFDDTFEKN LSSVRQVKEI

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against NLK. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NLK

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 527 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Nemo-Like Kinase

Target: Nemo-Like Kinase (NLK)

Alternative Name: NLK (NLK Products)

Synonyms: Amf antibody, Gpi1 antibody, AI194375 antibody, nlk antibody, nlkb antibody, zgc:111875 antibody, Xnlk antibody, nlk1 antibody, nlk2 antibody, NLK antibody, nemo-like antibody, RGD1561602 antibody, glucose-6-phosphate isomerase antibody, nemo like kinase antibody, nemo-like kinase, type 1 antibody, nemo-like kinase L homeolog antibody, Nemo-Like kinase antibody, nemo-like kinase antibody, Gpi antibody, NLK antibody, Nlk antibody, nlk1 antibody, nlk.L antibody, nlk antibody, nemo-like antibody

Background: NLK has a role in cell fate determination, required for differentiation of bone marrow stromal cells. NLK acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. NLK may suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP By similarity.NLK is involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
Alias Symbols: DKFZp761G1211, FLJ21033
Protein Interaction Partner: KRTAP5-6, KRTAP10-3, KRTAP4-2, QRICH1, ZHX3, KRTAP5-9, GRN, TP53, MDM2, STAT5A, LEF1, CNOT2, SMAD4, KPNB1, BACH1, TNKS1BP1, FAM222A, C2orf44, CEP97, RNF219, FAM222B, CNOT11, NLK, PASK, SHANK2, UBAP2L, CCP110, TRPS1, TLE3, SKI, RANGAP1, RANBP2, PKM, CNOT3,
Protein Size: 527

Molecular Weight: 58 kDa

Gene ID: 51701

NCBI Accession: NM_016231, NP_057315

UniProt: Q9UBE8

Pathways: Ubiquitin Proteasome Pathway