MTRR antibody (N-Term)

Catalog No. ABIN2787324

Product Details anti-MTRR Antibody

Target: MTRR (5-Methyltetrahydrofolate-Homocysteine Methyltransferase Reductase (MTRR))

Binding Specificity: N-Term

Reactivity: Human, Dog, Mouse, Rabbit, Rat, Cow, Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTRR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: YDLKTETAPL VVVVSTTGTG DPPDTARKFV KEIQNQTLPV DFFAHLRYGL

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MTRR. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MTRR

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 725 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MTRR antibody (ABIN2787324)

Richard, Desviat, Ugarte, Pérez: "Oxidative stress and apoptosis in homocystinuria patients with genetic remethylation defects." in: Journal of cellular biochemistry, Vol. 114, Issue 1, pp. 183-91, (2012) (PubMed).

Target Details for MTRR

Target: MTRR (5-Methyltetrahydrofolate-Homocysteine Methyltransferase Reductase (MTRR))

Alternative Name: MTRR (MTRR Products)

Background: Methionine is an essential amino acid required for protein synthesis and one-carbon metabolism. Its synthesis is catalyzed by the enzyme methionine synthase. Methionine synthase eventually becomes inactive due to the oxidation of its cob(I)alamin cofactor. The protein encoded by this gene regenerates a functional methionine synthase via reductive methylation. It is a member of the ferredoxin-NADP(+) reductase (FNR) family of electron transferases. Patients of the cbl-E complementation group of disorders of folate/cobalamin metabolism are defective in reductive activation of methionine synthase. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Alias Symbols: MGC129643, MSR, cblE
Protein Interaction Partner: GMCL1, PAPSS1, HIST1H2AG, FH, ELAVL1, UBC, MMAB,
Protein Size: 725

Molecular Weight: 80 kDa

Gene ID: 4552

NCBI Accession: NM_024010, NP_076915

UniProt: Q9UBK8

Pathways: Methionine Biosynthetic Process