PPP4C antibody (Middle Region)

Catalog No. ABIN2787719

Product Details anti-PPP4C Antibody

Target: PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Guinea Pig, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP4C antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: TVLTVWSAPN YCYRCGNVAA ILELDEHLQK DFIIFEAAPQ ETRGIPSKKP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 79%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against PPP4C. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PPP4C

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 307 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PPP4C

Target: PPP4C (Protein Phosphatase 4, Catalytic Subunit (PPP4C))

Alternative Name: PPP4C (PPP4C Products)

Synonyms: pp4 antibody, ppx antibody, pp4c antibody, pph3 antibody, MGC75928 antibody, PP4C-A antibody, gc:56413 antibody, ppp4c antibody, wu:fd05e08 antibody, zgc:56413 antibody, PP4 antibody, PP4C antibody, PPH3 antibody, PPP4 antibody, PPX antibody, 1110002D08Rik antibody, AU016079 antibody, Ppx antibody, protein phosphatase 4 catalytic subunit antibody, protein phosphatase 4, catalytic subunit a antibody, protein phosphatase 4, catalytic subunit antibody, protein phosphatase 4 catalytic subunit S homeolog antibody, ppp4c antibody, ppp4ca antibody, PPP4C antibody, Ppp4c antibody, ppp4c.S antibody

Background: PPP4C is a protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDC2/Cdk1 phosphorylation sites and negatively regulates CDC2/Cdk1 activity in interphase.
Alias Symbols: PP4, PPH3, PPX, PP4C, PPP4
Protein Interaction Partner: SUMO2, IKBKG, MAP4K1, BAG6, TCP1, UBC, CAND1, CUL3, Igbp1, TRIM28, ELAVL1, TK1, PPP2R5D, PPP2R4, PPP2R2B, PPP2R1A, EEF1A1, CCT6A, SMEK2, PPP2R2D, SMEK1, ATAD3A, PPME1, CCT5, CCT8, CCT2, CCT4, CCT7, PPP4R1, PLOD3, CCDC6, TUBA3C, CCT3, CCT6P1, GLYATL3, PPP4
Protein Size: 307

Molecular Weight: 34 kDa

Gene ID: 5531

NCBI Accession: NM_002720, NP_002711

UniProt: P60510