UCHL3 antibody (Middle Region)

Catalog No. ABIN2788014

Product Details anti-UCHL3 Antibody

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Mouse, Dog, Horse, Cow, Rabbit, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERAKFLEN YDAIRVTHET

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 83%

Characteristics: This is a rabbit polyclonal antibody against Uchl3. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Mouse Uchl3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 230 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for UCHL3

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Alternative Name: Uchl3 (Uchl3 Products)

Synonyms: UCH-L3 antibody, UCH-6 antibody, RGD1561196 antibody, im:6908825 antibody, zgc:109963 antibody, uchl4 antibody, ubiquitin C-terminal hydrolase L3 antibody, ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) antibody, ubiquitin C-terminal hydrolase L3 L homeolog antibody, UCHL3 antibody, Uchl3 antibody, uchl3 antibody, uchl3.L antibody

Background: Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Uchl3 has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Uchl3 deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Uchl3 indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance. Uchl3 may be involved in working memory and can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome.
Alias Symbols: -
Protein Interaction Partner: Nedd8,
Protein Size: 230

Molecular Weight: 26 kDa

Gene ID: 50933

NCBI Accession: NM_016723, NP_057932

UniProt: Q9JKB1

Pathways: Feeding Behaviour, Positive Regulation of fat Cell Differentiation