USP20 antibody (Middle Region)

Catalog No. ABIN2788022

Product Details anti-USP20 Antibody

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Dog, Rabbit, Rat, Horse, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP20 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PAEAQPPSPR SSSPCRTPEP DNDAHLRSSS RPCSPVHHHE GHAKLSSSPP

Predicted Reactivity: Cow: 92%, Dog: 92%, Guinea Pig: 100%, Horse: 92%, Human: 100%, Mouse: 85%, Rabbit: 100%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against USP20. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human USP20

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 913 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for USP20

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Alternative Name: USP20 (USP20 Products)

Synonyms: USP20 antibody, vdu2 antibody, lsfr3a antibody, LSFR3A antibody, VDU2 antibody, hVDU2 antibody, 1700055M05Rik antibody, AI467231 antibody, Vdu2 antibody, im:7151955 antibody, zgc:63661 antibody, universal stress protein antibody, ubiquitin specific peptidase 20 antibody, ubiquitin specific peptidase 20 L homeolog antibody, RR_RS15875 antibody, USP20 antibody, usp20 antibody, Usp20 antibody, usp20.L antibody

Background: USP20 is a deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling.USP20 acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). USP20 plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. USP20 deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity.USP20 mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Alias Symbols: KIAA1003, LSFR3A, VDU2
Protein Interaction Partner: MDFI, MAGEA11, TRIM23, ALAS1, FATE1, BEND5, SPRY2, VAPB, CCDC36, BEND7, RAD17, NEURL4, USP33, USP20, CCP110, ADRB2, PJA1, USP28, USP21, USP15, PLA2G2A, UBC, VHL, HIF1A, VAPA, HERC2P2, PLEKHA7, SYTL4, GPT2, POLDIP3, RGPD5, ATAD3B, MAP7D1, LRRC59, ZNF770, S
Protein Size: 913

Molecular Weight: 102 kDa

Gene ID: 10868

NCBI Accession: NM_006676, NP_006667

UniProt: Q9Y2K6

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling