HLA-DOB antibody (N-Term)

Catalog No. ABIN2788876

Product Details anti-HLA-DOB Antibody

Target: HLA-DOB (Major Histocompatibility Complex, Class II, DO beta (HLA-DOB))

Binding Specificity: N-Term

Reactivity: Human, Cow, Dog, Horse, Pig, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-DOB antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LTRLDSSMTQ GTDSPEDFVI QAKADCYFTN GTEKVQFVVR FIFNLEEYVR

Predicted Reactivity: Cow: 79%, Dog: 93%, Horse: 93%, Human: 100%, Pig: 93%, Rabbit: 86%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against HLA-DOB. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human HLA-DOB

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HLA-DOB

Target: HLA-DOB (Major Histocompatibility Complex, Class II, DO beta (HLA-DOB))

Alternative Name: HLA-DOB (HLA-DOB Products)

Synonyms: DOB antibody, major histocompatibility complex, class II, DO beta antibody, HLA-DOB antibody, PATR-DOB antibody

Background: HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.
Alias Symbols: DOB
Protein Interaction Partner: HLA-DMB, CD82, CD63,
Protein Size: 273

Molecular Weight: 30 kDa

Gene ID: 3112

NCBI Accession: NM_002120, NP_002111

UniProt: Q5QNS2

Pathways: Cancer Immune Checkpoints