SPAM1 antibody (C-Term)
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- Target See all SPAM1 Antibodies
- SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SPAM1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- CYSTLSCKEK ADVKDTDAVD VCIADGVCID AFLKPPMETE EPQIFYNASP
- Predicted Reactivity
- Human: 100%
- Characteristics
- This is a rabbit polyclonal antibody against SPAM1. It was validated on Western Blot.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the C-terminal region of Human SPAM1
- Top Product
- Discover our top product SPAM1 Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 509 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))
- Alternative Name
- SPAM1 (SPAM1 Products)
- Synonyms
- AV039194 antibody, Ph-20 antibody, PH-20 antibody, PH20 antibody, HYA1 antibody, HYAL1 antibody, HYAL3 antibody, HYAL5 antibody, SPAG15 antibody, Spam antibody, XEH1 antibody, spam1 antibody, im:6902904 antibody, zgc:158668 antibody, sperm adhesion molecule 1 antibody, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) L homeolog antibody, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) antibody, Spam1 antibody, SPAM1 antibody, spam1.L antibody, spam1 antibody
- Background
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Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1, another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: HYA1, HYAL1, HYAL3, HYAL5, MGC26532, PH-20, PH20, SPAG15
Protein Size: 509 - Molecular Weight
- 58 kDa
- Gene ID
- 6677
- NCBI Accession
- NM_153189, NP_694859
- UniProt
- P38567
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