zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody (PE)

Antigen: zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)

Synonyms: SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70

Clonality: Monoclonal (1E7-2)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN279079

Additional Information

Alternative name: ZAP-70

Isotype: IgG1, kappa chain

Clone: 1E7-2

Specificity: The 1E7.2 antibody reacts with human and mouse ZAP -70, the TCRzeta -associated protein -70. ZAP -70 is a cytosolic protein tyrosine kinase (PTK) and a member of the Syk family of proteins. It is expressed in T and NK cells and is required for TCR signaling and development. ZAP -70 interacts with the TCR complex by binding to tyrosine -phosphorylated immunoreceptor tyrosine -based activation motifs (ITAMs) present in the invariant subunits of the TCR complex. Following activation, ZAP -70 is phosphorylated on several tyrosine residues by two mechanisms; an autophosphorylation and a transphosphorylation by the Src family tyrosine kinase Lck1 -3. Tyrosine phosphorylation of ZAP -70 correlates to its increased kinase activity and triggers downstream signaling events. Mutations in ZAP -70 have been shown to result in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. 1E7.2 was generated against a KLH -peptide sequence corresponding to the human ZAP -70 amino acid residues 282 -307. While ZAP -70 is normally expressed in T and NK cells, several recent studies have also shown high correlation of ZAP -70 positive expression with mutated IgVH expression in B -chronic lymphocytic leukemia (CCL). In conclusion, the expression of ZAP -70, which can be measured by intracellular flow cytometry, may serve as a prognostic marker for B -CLL., Human, Mouse

Application Details

Application Notes: This 1E7.2 antibody is offered in 2 formats: - µg size: has been tested by intracellular flow cytometric analysis of mouse thymocytes and human Jurkat cells. This can be used at less than or equal to 1 µg per million cells in a 100 µl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre -titrated and tested by intracellular flow cytometric analysis of mouse thymocytes and human Jurkat cells. This can be used at 20 µl (0.5 µg) per million cells in a 100 µl total staining volume. The pre -titrated test size contains BSA and/or gelatin for protein stabilization. Nothing found

Buffer: Phosphate buffer pH 7.2,150 mM NaCl, 0.09% NaN3

Storage: Store at 4 °C.DO NOT FREEZE.LIGHT -SENSITIVE MATERIAL.

Research Area: Signaling, Tags/Labels, Immunology, Cancer

Restrictions: For Research Use only

Images

Staining of permeabilized C57Bl/6 thymocytes (left) and permeabilized Jurkat (right) cells with 0.5 µg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4719) (open histogram) or 0.5 µg of PE 1E7.2 (colored histogram). Total cells were used for analysis.

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody (PE) (Image 2)