ASAP2 antibody (N-Term)

Catalog No. ABIN2790909

Product Details anti-ASAP2 Antibody

Target: ASAP2 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 2 (ASAP2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Horse, Dog, Rabbit, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASAP2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LSTDLHTIKQ AQDEERRQLI QLRDILKSAL QVEQKEDSQI RQSTAYSLHQ

Predicted Reactivity: Dog: 100%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 78%, Rat: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against ASAP2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human ASAP2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1006 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ASAP2

Target: ASAP2 (ArfGAP with SH3 Domain, Ankyrin Repeat and PH Domain 2 (ASAP2))

Alternative Name: ASAP2 (ASAP2 Products)

Background: This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport.
Alias Symbols: AMAP2, CENTB3, DDEF2, PAG3, PAP, Pap-alpha, SHAG1
Protein Interaction Partner: SH3KBP1, MAP1LC3A, CTTN, Asap1, IKBKG, UBC, SUMO1, ELAVL1, ITSN1, THSD7A, GRB2, PACSIN3, PXN, BIN1, PTK2B, RAN, ARF6, REPS2, ARF5, ARF1, SRC, TBRG4,
Protein Size: 1006

Molecular Weight: 112 kDa

Gene ID: 8853

NCBI Accession: NM_003887, NP_003878

UniProt: O43150