IL-13 antibody (PE)

Antigen: IL-13

Clonality: Monoclonal (E13A)

Host: Please enquire

Reactivity: Mouse (Murine)

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN279133

Additional Information

Isotype: IgG1, kappa chain

Clone: E13A

Specificity: The eBio13A antibody reacts with mouse IL -13. IL -13 is a cytokine produced mainly by Th2 cells, but also by antigen -primed CD8 T cells. IL -13 has a strong involvement in allergic inflammation and parasitic clearing and in cancer models has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL -13 is found to play a role in isotype switching in B cells. IL -13 is implicating in down modulating macrophage activity, through the reduction of pro -inflammatory cytokines (IL -1, IL -6, IL -8, IL -10, IL -12), Mouse

Application Details

Application Notes: This eBio13A antibody is offered in 2 formats: - µg size: has been tested intracellular staining of cultured mouse splenocytes. This can be used at less than or equal to 0.25 µg per million cells in a 100 µl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre -titrated and tested intracellular staining of cultured mouse splenocytes. This can be used at 20 µl (0.125 µg) per million cells in a 100 µl total staining volume. The pre -titrated test size contains BSA and/or gelatin for protein stabilization. The eBio13A antibody has been reported useful for ELISA and ELISPOT capture, as well as intracellular staining for flow cytometric analysis.

Buffer: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3

Storage: Store at 4 °C.DO NOT FREEZE.LIGHT -SENSITIVE MATERIAL.

Research Area: Cytokines, Immunology, Virology, Inflammation, Cancer

Restrictions: For Research Use only

Images

BALB/c splenocytes were cultured for 3 days with plate -bound anti -mouse CD3e (clone 145 -2C11, cat. 16 -0031), soluble anti -mouse CD28 (clone 37.51, cat. 16 -0281), recombinant mouse IL -2 (cat. 14-8021), and recombinant mouse IL -4 (cat. 14-8041). Cells were then harvested and restimulated with PMA/Ionomycin in the presence of monensin for 5 hours. Following restimulation, cells were harvested, surface stained with FITC anti -mouse CD4 (clone RM4 -5, cat. 11 -0042), fixed and permeabilized, then stained with 0.125 µg PE eBio13A. (Left Plot) The ligand blocking control demonstrates that pre -blocking the PE conjugated eBio13A antibody with 1µg of recombinant mouse IL -13 (cat. 14-8131) prior to staining abrogates specific mouse IL -13 staining (Right Plot).

anti-IL-13 antibody (PE) (Image 2)