RAD9A antibody (C-Term)

Catalog No. ABIN2792162

Product Details anti-RAD9A Antibody

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Binding Specificity: C-Term

Reactivity: Human, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD9A antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SLSPGPQPPK SPGPHSEEED EAEPSTVPGT PPPKKFRSLF FGSILAPVRS

Predicted Reactivity: Horse: 84%, Human: 100%

Characteristics: This is a rabbit polyclonal antibody against RAD9A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RAD9A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 391 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RAD9A

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Alternative Name: RAD9A (RAD9A Products)

Synonyms: RAD9 antibody, Rad9 antibody, rad9a antibody, MGC89062 antibody, XRad9 antibody, rad9 antibody, RAD9 checkpoint clamp component A antibody, RAD9 checkpoint clamp component B antibody, RAD9 checkpoint clamp component A L homeolog antibody, RAD9A antibody, Rad9a antibody, rad9a antibody, rad9b antibody, rad9a.L antibody

Background: RAD9A is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]).[supplied by OMIM].
Alias Symbols: RAD9
Protein Interaction Partner: ATRX, BCL2L1, CLSPN, RAD17, MSH3, MSH2, MSH6, FEM1B, COPS5, UBC, PCNA, SF3B3, DNMT1, SETMAR, ATR, TOPBP1, HUS1B, RAD9B, ITSN2, ATAD5, DNAJC7, ATM, CHEK2, HUS1, HDAC1, AR, ABL1, BCL2, MLH1, FEN1, RPA2, RPA1, RAD1, NR3C1,
Protein Size: 391

Molecular Weight: 42 kDa

Gene ID: 5883

NCBI Accession: NM_004584, NP_004575

UniProt: Q99638

Pathways: Positive Regulation of Response to DNA Damage Stimulus