p107 antibody (N-Term)

Catalog No. ABIN2792553

Product Details anti-p107 Antibody

Target: p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Horse, Pig, Cow, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p107 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: FLDIFQNPYE EPPKLPRSRK QRRIPCSVKD LFNFCWTLFV YTKGNFRMIG

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 85%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 92%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against RBL1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RBL1

Application Details

Application Notes: WB Suggested Anti-RBL1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: COLO205 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1014 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for p107

Target: p107 (RBL1) (Retinoblastoma-Like 1 (p107) (RBL1))

Alternative Name: RBL1 (RBL1 Products)

Synonyms: p107 antibody, prb1 antibody, fj58e06 antibody, wu:fj58e06 antibody, CP107 antibody, PRB1 antibody, AW547426 antibody, retinoblastoma-like 1 L homeolog antibody, RB transcriptional corepressor like 1 antibody, retinoblastoma-like 1 (p107) antibody, retinoblastoma-like 1 antibody, rbl1.L antibody, RBL1 antibody, rbl1 antibody, Rbl1 antibody

Background: RBL1 is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: CP107, MGC40006, PRB1, p107
Protein Interaction Partner: DYRK1B, DYRK1A, CDK2, MAGEA11, E2F1, AR, UBC, RBBP8, E2F4, PLSCR1, NUCB1, LAMB2, NR4A1, GOLGA2, FN1, EPHA2, CDK6, CDK4, AOX1, SP1, SNRPD3, DGKZ, ID2, RINT1, E2F3, E2F2, PPP1CA, TOP1, SMARCA4, CCNA2, LIN9, LIN54, LIN37, MYBL2, MAPK6, IRF3, RBL2, DHX30, NR2
Protein Size: 1014

Molecular Weight: 115 kDa

Gene ID: 5933

NCBI Accession: NM_183404, NP_899662

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases