SMAD6 antibody (N-Term)
-
- Target See all SMAD6 Antibodies
- SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))
-
Binding Specificity
- N-Term
-
Reactivity
- Human, Cow, Dog, Pig
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This SMAD6 antibody is un-conjugated
-
Application
- Western Blotting (WB)
- Sequence
- GAPRDASDPL AGAALEPAGG GRSREARSRL LLLEQELKTV TYSLLKRLKE
- Cross-Reactivity
- Human
- Predicted Reactivity
- Cow: 85%, Dog: 91%, Human: 100%, Pig: 85%
- Characteristics
- This is a rabbit polyclonal antibody against SMAD6. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD6
- Top Product
- Discover our top product SMAD6 Primary Antibody
-
-
- Application Notes
-
WB Suggested Anti-SMAD6 Antibody Titration: 0.2-1 µg/mL
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator. - Comment
-
Antigen size: 496 AA
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
-
- Target
- SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))
- Alternative Name
- SMAD6 (SMAD6 Products)
- Synonyms
- madh6 antibody, madh7 antibody, Xsmad6 antibody, hst17432 antibody, AOVD2 antibody, HsT17432 antibody, MADH6 antibody, MADH7 antibody, Madh6 antibody, b2b390Clo antibody, Smad6-like antibody, smad6 antibody, wu:fb22g05 antibody, SMAD family member 6 antibody, SMAD family member 6a antibody, smad6 antibody, SMAD6 antibody, Smad6 antibody, smad6a antibody
- Background
-
SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members, has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.
Alias Symbols: HsT17432, MADH6, MADH7
Protein Interaction Partner: ITCH, SMURF2, BPIFA1, NEDD4L, WWP2, WWP1, USP15, USP11, PIGR, NASP, LYZ, KPNA2, CTBP2, CTBP1, UBC, ACVR1, UBE2O, BAMBI, STAMBP, ACTR1B, TGFBR1, MYD88, SMAD1, BMPR2, BMPR1B, BMPR1A, ACVR2B, ACVR1B, SMURF1, PELI1, CUL2, ELAVL1, SMAD5, RPS6KA5, TSC22D1, MAPK
Protein Size: 496 - Molecular Weight
- 53 kDa
- Gene ID
- 4091
- NCBI Accession
- NM_005585, NP_005576
- UniProt
- O43541
- Pathways
- Chromatin Binding
-