SMAD6 antibody (N-Term)

Catalog No. ABIN2792563

Product Details anti-SMAD6 Antibody

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Binding Specificity: N-Term

Reactivity: Human, Cow, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD6 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GAPRDASDPL AGAALEPAGG GRSREARSRL LLLEQELKTV TYSLLKRLKE

Cross-Reactivity: Human

Predicted Reactivity: Cow: 85%, Dog: 91%, Human: 100%, Pig: 85%

Characteristics: This is a rabbit polyclonal antibody against SMAD6. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD6

Application Details

Application Notes: WB Suggested Anti-SMAD6 Antibody Titration: 0.2-1 µg/mL
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 496 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SMAD6

Target: SMAD6 (SMAD, Mothers Against DPP Homolog 6 (SMAD6))

Alternative Name: SMAD6 (SMAD6 Products)

Synonyms: madh6 antibody, madh7 antibody, Xsmad6 antibody, hst17432 antibody, AOVD2 antibody, HsT17432 antibody, MADH6 antibody, MADH7 antibody, Madh6 antibody, b2b390Clo antibody, Smad6-like antibody, smad6 antibody, wu:fb22g05 antibody, SMAD family member 6 antibody, SMAD family member 6a antibody, smad6 antibody, SMAD6 antibody, Smad6 antibody, smad6a antibody

Background: SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members, has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.
Alias Symbols: HsT17432, MADH6, MADH7
Protein Interaction Partner: ITCH, SMURF2, BPIFA1, NEDD4L, WWP2, WWP1, USP15, USP11, PIGR, NASP, LYZ, KPNA2, CTBP2, CTBP1, UBC, ACVR1, UBE2O, BAMBI, STAMBP, ACTR1B, TGFBR1, MYD88, SMAD1, BMPR2, BMPR1B, BMPR1A, ACVR2B, ACVR1B, SMURF1, PELI1, CUL2, ELAVL1, SMAD5, RPS6KA5, TSC22D1, MAPK
Protein Size: 496

Molecular Weight: 53 kDa

Gene ID: 4091

NCBI Accession: NM_005585, NP_005576

UniProt: O43541

Pathways: Chromatin Binding