CTCF antibody (CCCTC-Binding Factor (Zinc Finger Protein)) (N-Term)

Details for Product anti-CTCF Antibody No. ABIN2792571, Supplier: Log in to see
Antigen
  • im:7152666
  • CTCF
  • xctcf
  • AW108038
  • CCCTC-binding factor (zinc finger protein)
  • CCCTC-binding factor
  • CTCF
  • ctcf
  • LOC100224119
  • Ctcf
Epitope
N-Term
14
7
6
5
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
89
45
30
12
10
8
6
6
4
4
3
2
2
1
1
1
Host
Rabbit
60
27
6
1
1
Clonality
Polyclonal
Application
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
79
29
27
16
14
11
9
6
3
2
1
1
Options
Supplier
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human CTCF
Sequence GELPPQEDPS WQKDPDYQPP AKKTKKTKKS KLRYTEEGKD VDVSVYDFEE
Cross-Reactivity Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)
Predicted Reactivity Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against CTCF. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name CTCF (CTCF Antibody Abstract)
Background CTCF is a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in CTCF have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.
Protein Interaction Partner: ZMYM4, UBC, EED, BMI1, KANSL1, CDK19, ZMYM2, CAND1, ZBTB33, TFAP4, SUMO1, SUMO2, SUMO3, SIRT7, TAF3, SMC3, POLR2A, SUZ12, Ctcf, HIST2H2AC, TOP2A, TAF1, NPM1, LMNA, KPNA4, KPNA2, H2AFZ, PARP1, TRB, RXRA, SMAD3, SIN3A, SMC1A, RAD21, YY1, THRB, SMAD4, HMGB1,
Protein Size: 727
Molecular Weight 83 kDa
Gene ID 10664
NCBI Accession NM_006565, NP_006556
UniProt P49711
Application Notes WB Suggested Anti-CTCF Antibody Titration: 0.2-1 µg/mL
Positive Control: Transfected 293T.
Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 727 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (N-Term) antibody (ABIN2792571) WB Suggested Anti-CTCF Antibody Titration: 0.2-1 ug/ml
Positive Control:...
Chromatin Immunoprecipitation (ChIP) image for anti-CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (N-Term) antibody (ABIN2792571) Chromatin Immunoprecipitation (ChIP) Using CTCF Antibody - N-terminal region (P100757...
Background publications Defossez, Kelly, Filion, Pérez-Torrado, Magdinier, Menoni, Nordgaard, Daniel, Gilson: "The human enhancer blocker CTC-binding factor interacts with the transcription factor Kaiso." in: The Journal of biological chemistry, Vol. 280, Issue 52, pp. 43017-23, 2005 (PubMed).

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