TAF1A antibody (C-Term)

Catalog No. ABIN2792590

Product Details anti-TAF1A Antibody

Target: TAF1A (TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, A, 48kDa (TAF1A))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Horse, Rabbit, Pig, Dog, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: CEKAFVAGLL LGKGCRYFRY ILKQDHQILG KKIKRMKRSV KKYSIVNPRL

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 78%, Dog: 85%, Horse: 85%, Human: 100%, Mouse: 85%, Pig: 85%, Rabbit: 85%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against TAF1A. It was validated on Western Blot using a cell lysate as a positive control.

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TAF1A

Application Details

Application Notes: WB Suggested Anti-TAF1A Antibody Titration: 0.2-1 µg/mL
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 336 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TAF1A antibody (ABIN2792590)

Di Pietro, Rapisarda, Amico, Bonaiuto, Viola, Scalia, Motta, Amato, Engel, Messina, Sichel, Grzeschik, Purrello: "Genomic localization of the human genes TAF1A, TAF1B and TAF1C, encoding TAF(I)48, TAF(I)63 and TAF(I)110 subunits of class I general transcription initiation factor SL1." in: Cytogenetics and cell genetics, Vol. 89, Issue 1-2, pp. 133-6, (2000) (PubMed).

Target Details for TAF1A

Target: TAF1A (TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, A, 48kDa (TAF1A))

Alternative Name: TAF1A (TAF1A Products)

Synonyms: TAFI48 antibody, mTAFI48 antibody, MGC:17061 antibody, RAFI48 antibody, SL1 antibody, TATA-box binding protein associated factor, RNA polymerase I, A antibody, TATA-box binding protein associated factor, RNA polymerase I subunit A antibody, Taf1a antibody, TAF1A antibody

Background: Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. TAF1A encodes the smallest SL1-specific TAF.
Alias Symbols: SL1, RAFI48, TAFI48, MGC:17061
Protein Interaction Partner: C11orf57, JMJD6, TBP, Taf1c, Taf1b, BKRF1, UBC, TAF12, TAF1D, HIST1H4A, HIST1H3A, HIST2H2BE, HIST2H2AC, ESR1, SET, TAF1A, RUNX2, TP53, CD3EAP, HIST4H4, HIST3H3, ANP32A, UBTF, RRN3, MYC,
Protein Size: 336

Molecular Weight: 40 kDa

Gene ID: 9015

NCBI Accession: NM_139352, NP_647603

UniProt: Q9NWA1