GTF2E1 antibody (N-Term)

Catalog No. ABIN2792622

Product Details anti-GTF2E1 Antibody

Target: GTF2E1 (General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2E1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DHMRRRIETD ERDSTNRASF KCPVCSSTFT DLEANQLFDP MTGTFRCTFC

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 92%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against GTF2E1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GTF2E1

Application Details

Application Notes: WB Suggested Anti-GTF2E1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: 721_B cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 439 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GTF2E1

Target: GTF2E1 (General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1))

Alternative Name: GTF2E1 / TF2E1 (GTF2E1 Products)

Synonyms: 2610024P03Rik antibody, 56kDa antibody, AI303800 antibody, FE antibody, TFIIE-A antibody, DDBDRAFT_0168201 antibody, DDBDRAFT_0168202 antibody, DDBDRAFT_0231096 antibody, DDB_0168201 antibody, DDB_0168202 antibody, DDB_0231096 antibody, DDBDRAFT_0217176 antibody, DDBDRAFT_0217177 antibody, DDBDRAFT_0266401 antibody, DDB_0217176 antibody, DDB_0217177 antibody, DDB_0266401 antibody, TF2E1 antibody, si:ch211-194d6.2 antibody, gtf2e1 antibody, tfiiealpha antibody, general transcription factor II E, polypeptide 1 (alpha subunit) antibody, general transcription factor IIE subunit 1 antibody, transcription factor IIE antibody, general transcription factor IIE subunit 2 antibody, general transcription factor IIE, polypeptide 2, beta 34kDa antibody, general transcription factor IIE, polypeptide 1, alpha antibody, general transcription factor IIE subunit 1 L homeolog antibody, Gtf2e1 antibody, gtf2e1-1 antibody, gtf2e1-2 antibody, GTF2E2 antibody, GTF2E1 antibody, gtf2e1 antibody, gtf2e1.L antibody

Background: GTF2E1 belongs to the TFIIE alpha subunit family. It recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Alias Symbols: FE, TF2E1, TFIIE-A
Protein Interaction Partner: UBC, AARSD1, ACBD3, XPO5, SH3GLB2, OXR1, NSUN2, RPUSD2, SNF8, EFTUD2, TTC1, SH3GL1, SARS, HARS, SRPK2, TCEA1, GTF2H1, GTF2E2, CDK7, APP, tat, GTF2F1, ELAVL1, ZSCAN1, ZBTB9, TRIM15, PCBD2, TRIM29, CBFA2T2, IKBKG, ATF7IP, TAF6, GTF2B, TRIM24, SND1, GTF2H4,
Protein Size: 439

Molecular Weight: 49 kDa

Gene ID: 2960

NCBI Accession: NM_005513, NP_005504

UniProt: P29083