NFKBIL2 antibody (Middle Region)

Catalog No. ABIN2792627

Product Details anti-NFKBIL2 Antibody

Target: NFKBIL2 (Tonsoku-Like, DNA Repair Protein (NFKBIL2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIL2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RAIIHVSLAT TLGDMKDHHG AVRHYEEELR LRSGNVLEEA KTWLNIALSR

Cross-Reactivity: Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Dog: 84%, Human: 100%, Mouse: 83%, Rat: 83%

Characteristics: This is a rabbit polyclonal antibody against NFKBIL2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFKBIL2

Application Details

Application Notes: WB Suggested Anti-NFKBIL2 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: 293T cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1219 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NFKBIL2 antibody (ABIN2792627)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for NFKBIL2

Target: NFKBIL2 (Tonsoku-Like, DNA Repair Protein (NFKBIL2))

Alternative Name: NFKBIL2 / IKBR (NFKBIL2 Products)

Synonyms: IKBR antibody, NFKBIL2 antibody, 2810439M11Rik antibody, Nfkbil2 antibody, im:7145273 antibody, nfkbil2 antibody, tonsl antibody, tonsoku like, DNA repair protein antibody, tonsoku-like, DNA repair protein antibody, TONSL antibody, Tonsl antibody, tonsl antibody

Background: NFKBIL2 is thought to be a negative regulator of NF-kappa-B mediated transcription. It may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus.The protein encoded by this gene is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus.
Alias Symbols: FLJ40087, IKBR, NFKBIL2
Protein Interaction Partner: HIST3H3, MCM6, MCM2, MMS22L, ASF1B, ASF1A, UBC, MYC, MAU2, SLC25A5, SUPT16H, SUGT1, AIFM1, HIST1H2BL, XRCC5, SSRP1, SLC25A3, TONSL, MCM4, HIST1H2AE, HIST1H1B, HIST1H1C, XRCC6, GSK3B, MCM7, MCM5, MCM3, EWSR1,
Protein Size: 1219

Molecular Weight: 133 kDa

Gene ID: 4796

NCBI Accession: NM_013432, NP_038460

UniProt: Q96HA7

Pathways: Maintenance of Protein Location