GTF2E1 antibody (C-Term)

Catalog No. ABIN2792638

Product Details anti-GTF2E1 Antibody

Target: GTF2E1 (General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1))

Binding Specificity: C-Term

Reactivity: Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: VADDPIVMVA GRPFSYSEVS QRPELVAQMT PEEKEAYIAM GQRMFEDLFE

Cross-Reactivity: Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against GTF2E1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GTF2E1

Application Details

Application Notes: WB Suggested Anti-GTF2 E1 Antibody Titration: 0.2-1 µg/mL
Positive Control: Daudi cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 439 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GTF2E1

Target: GTF2E1 (General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1))

Alternative Name: GTF2E1 / TF2E1 (GTF2E1 Products)

Synonyms: 2610024P03Rik antibody, 56kDa antibody, AI303800 antibody, FE antibody, TFIIE-A antibody, DDBDRAFT_0168201 antibody, DDBDRAFT_0168202 antibody, DDBDRAFT_0231096 antibody, DDB_0168201 antibody, DDB_0168202 antibody, DDB_0231096 antibody, DDBDRAFT_0217176 antibody, DDBDRAFT_0217177 antibody, DDBDRAFT_0266401 antibody, DDB_0217176 antibody, DDB_0217177 antibody, DDB_0266401 antibody, TF2E1 antibody, si:ch211-194d6.2 antibody, gtf2e1 antibody, tfiiealpha antibody, general transcription factor II E, polypeptide 1 (alpha subunit) antibody, general transcription factor IIE subunit 1 antibody, transcription factor IIE antibody, general transcription factor IIE subunit 2 antibody, general transcription factor IIE, polypeptide 2, beta 34kDa antibody, general transcription factor IIE, polypeptide 1, alpha antibody, general transcription factor IIE subunit 1 L homeolog antibody, Gtf2e1 antibody, gtf2e1-1 antibody, gtf2e1-2 antibody, GTF2E2 antibody, GTF2E1 antibody, gtf2e1 antibody, gtf2e1.L antibody

Background: The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57,000 (TFIIE-alpha) and 34,000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation
Alias Symbols: FE, TF2E1, TFIIE-A
Protein Interaction Partner: UBC, AARSD1, ACBD3, XPO5, SH3GLB2, OXR1, NSUN2, RPUSD2, SNF8, EFTUD2, TTC1, SH3GL1, SARS, HARS, SRPK2, TCEA1, GTF2H1, GTF2E2, CDK7, APP, tat, GTF2F1, ELAVL1, ZSCAN1, ZBTB9, TRIM15, PCBD2, TRIM29, CBFA2T2, IKBKG, ATF7IP, TAF6, GTF2B, TRIM24, SND1, GTF2H4,
Protein Size: 439

Molecular Weight: 49 kDa

Gene ID: 2960

NCBI Accession: NM_005513, NP_005504

UniProt: P29083