ATF4 antibody (Middle Region)

Catalog No. ABIN2792651

Product Details anti-ATF4 Antibody

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF4 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LGSEVDITEG DRKPDYTAYV AMIPQCIKEE DTPSDNDSGI CMSPESYLGS

Cross-Reactivity: Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Predicted Reactivity: Dog: 78%, Human: 100%, Mouse: 90%, Pig: 81%, Rat: 90%

Characteristics: This is a rabbit polyclonal antibody against ATF4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATF4

Application Details

Application Notes: WB Suggested Anti-ATF4 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:1562500
Positive Control: Human Muscle.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 351 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Alternative Name: ATF4 (ATF4 Products)

Synonyms: CREB-2 antibody, CREB2 antibody, TAXREB67 antibody, TXREB antibody, ATF4 antibody, atf-4 antibody, creb-2 antibody, creb2 antibody, taxreb67 antibody, txreb antibody, atf4 antibody, wu:fj01c08 antibody, wu:fk30b04 antibody, zgc:85951 antibody, sb:eu681 antibody, wu:fb08f07 antibody, zgc:171702 antibody, Atf-4 antibody, C/ATF antibody, ATF4-I antibody, atf4-a antibody, atf4-b antibody, atf4-ii antibody, activating transcription factor 4 antibody, activating transcription factor 4 L homeolog antibody, activating transcription factor 4a antibody, activating transcription factor 4b antibody, activating transcription factor 4 S homeolog antibody, ATF4 antibody, atf4 antibody, Atf4 antibody, atf4.L antibody, atf4a antibody, atf4b antibody, atf4.S antibody

Background: ATF4s a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.
Alias Symbols: CREB-2, CREB2, TAXREB67, TXREB
Protein Interaction Partner: GPS2, GOLGA1, EP300, CEBPG, GOLGA8F, GOLGA8EP, GCC1, TRIB3, CCDC106, LDOC1, FBXW11, NDC80, BTRC, BFSP2, DDIT3, CREBBP, NEK6, JUN, FOS, ATF2, CEBPE, CEBPA, ATF4, ATF3, BATF2, CREBZF, BATF3, BATF, CREB3, MAFB, FOSL1, NFE2L2, NFE2, MAF, UBC, TGM2, RPS6, MAP2
Protein Size: 351

Molecular Weight: 38 kDa

Gene ID: 468

NCBI Accession: NM_001675, NP_001666

UniProt: P18848

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response