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OCT4 antibody (N-Term)

POU5F1 Reactivity: Human, Pig, Dog, Rabbit, Goat WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2792671
  • Target See all OCT4 (POU5F1) Antibodies
    OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
    Binding Specificity
    • 16
    • 14
    • 8
    • 7
    • 6
    • 6
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 119
    • 60
    • 30
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Pig, Dog, Rabbit, Goat
    Host
    • 107
    • 25
    • 1
    • 1
    Rabbit
    Clonality
    • 98
    • 35
    Polyclonal
    Conjugate
    • 95
    • 6
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This OCT4 antibody is un-conjugated
    Application
    • 112
    • 61
    • 32
    • 21
    • 17
    • 17
    • 15
    • 14
    • 13
    • 11
    • 9
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB)
    Sequence
    PCTVTPGAVK LEKEKLEQNP EESQDIKALQ KELEQFAKLL KQKRITLGYT
    Cross-Reactivity
    Cow (Bovine), Dog (Canine), Human, Pig (Porcine), Rabbit
    Predicted Reactivity
    Dog: 91%, Goat: 100%, Human: 100%, Pig: 84%, Rabbit: 92%
    Characteristics
    This is a rabbit polyclonal antibody against POU5F1. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Protein A purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the N terminal region of human POU5F1
    Top Product
    Discover our top product POU5F1 Primary Antibody
  • Application Notes
    WB Suggested Anti-POU5F1 Antibody Titration: 1.25 µg/mL
    ELISA Titer: 1:62500
    Positive Control: Jurkat cell lysate.
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 360 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Li, Moretto-Zita, Soncin, Wakeland, Wolfe, Leon-Garcia, Pandian, Pizzo, Cui, Nazor, Loring, Crum, Laurent, Parast: "BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ?Np63+ cytotrophoblast stem cell state." in: Development (Cambridge, England), Vol. 140, Issue 19, pp. 3965-76, (2013) (PubMed).

    Kaeffer, Legrand, Moyon, Frondas-Chauty, Billard, Guzman-Quevedo, Darmaun, Rozé: "Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells." in: PLoS ONE, Vol. 6, Issue 10, pp. e25562, (2011) (PubMed).

    Suo, Han, Wang, Zhang, Zhao, Zhao, Dai: "Oct4 pseudogenes are transcribed in cancers." in: Biochemical and biophysical research communications, Vol. 337, Issue 4, pp. 1047-51, (2005) (PubMed).

  • Target
    OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
    Alternative Name
    OCT3/4 (POU5F1 Products)
    Synonyms
    OCT3 antibody, OCT4 antibody, OTF-3 antibody, OTF3 antibody, OTF4 antibody, Oct-3 antibody, Oct-4 antibody, POU5F1 antibody, gp9 antibody, gp-9 antibody, oct4 antibody, pou2 antibody, POU-2 antibody, cb197 antibody, spg/pou2 antibody, chunp6868 antibody, wu:fd18d06 antibody, NF-A3 antibody, Oct-3/4 antibody, Oct3 antibody, Oct3/4 antibody, Oct4 antibody, Otf-3 antibody, Otf-4 antibody, Otf3 antibody, Otf3-rs7 antibody, Otf3g antibody, Otf4 antibody, POU class 5 homeobox 1 antibody, POU domain, class 5, transcription factor 3 antibody, POU domain, class 5, transcription factor 1 antibody, POU5F1 antibody, pou5f3 antibody, Pou5f1 antibody
    Background
    POU5F1 is a POU transcription factor expressed by early embryo cells and germ cells. It determines paracrine growth factor signaling from stem cells to the trophectoderm
    Alias Symbols: OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4
    Protein Interaction Partner: ETS2, CETN2, XPC, RAD23B, UBC, ITCH, FOXD3, WWP2, PSMD10, NCL, PURA, PPIA, POU5F1, YBX1, KRT19, KRT14, KRT10, KRT9, KRT2, KRT1, HNRNPK, HMGB1, HIST1H1T, GAPDH, FKBP7, EHMT2, AGR2, TUBB4A, EIF5B, TUBA1A, NR2F2, TRA2B, SRSF3, RPS25, RPS23, RPS19, RPL31, RPL
    Protein Size: 360
    Molecular Weight
    39 kDa
    Gene ID
    5460
    NCBI Accession
    NM_002701, NP_002692
    UniProt
    Q01860
    Pathways
    Stem Cell Maintenance
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