OCT4 antibody (N-Term)
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- Target See all OCT4 (POU5F1) Antibodies
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
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Binding Specificity
- N-Term
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Reactivity
- Human, Pig, Dog, Rabbit, Goat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This OCT4 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- PCTVTPGAVK LEKEKLEQNP EESQDIKALQ KELEQFAKLL KQKRITLGYT
- Cross-Reactivity
- Cow (Bovine), Dog (Canine), Human, Pig (Porcine), Rabbit
- Predicted Reactivity
- Dog: 91%, Goat: 100%, Human: 100%, Pig: 84%, Rabbit: 92%
- Characteristics
- This is a rabbit polyclonal antibody against POU5F1. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Protein A purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human POU5F1
- Top Product
- Discover our top product POU5F1 Primary Antibody
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- Application Notes
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WB Suggested Anti-POU5F1 Antibody Titration: 1.25 µg/mL
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator. - Comment
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Antigen size: 360 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ?Np63+ cytotrophoblast stem cell state." in: Development (Cambridge, England), Vol. 140, Issue 19, pp. 3965-76, (2013) (PubMed).
: "Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells." in: PLoS ONE, Vol. 6, Issue 10, pp. e25562, (2011) (PubMed).
: "Oct4 pseudogenes are transcribed in cancers." in: Biochemical and biophysical research communications, Vol. 337, Issue 4, pp. 1047-51, (2005) (PubMed).
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BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ?Np63+ cytotrophoblast stem cell state." in: Development (Cambridge, England), Vol. 140, Issue 19, pp. 3965-76, (2013) (PubMed).
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- Target
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
- Alternative Name
- OCT3/4 (POU5F1 Products)
- Synonyms
- OCT3 antibody, OCT4 antibody, OTF-3 antibody, OTF3 antibody, OTF4 antibody, Oct-3 antibody, Oct-4 antibody, POU5F1 antibody, gp9 antibody, gp-9 antibody, oct4 antibody, pou2 antibody, POU-2 antibody, cb197 antibody, spg/pou2 antibody, chunp6868 antibody, wu:fd18d06 antibody, NF-A3 antibody, Oct-3/4 antibody, Oct3 antibody, Oct3/4 antibody, Oct4 antibody, Otf-3 antibody, Otf-4 antibody, Otf3 antibody, Otf3-rs7 antibody, Otf3g antibody, Otf4 antibody, POU class 5 homeobox 1 antibody, POU domain, class 5, transcription factor 3 antibody, POU domain, class 5, transcription factor 1 antibody, POU5F1 antibody, pou5f3 antibody, Pou5f1 antibody
- Background
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POU5F1 is a POU transcription factor expressed by early embryo cells and germ cells. It determines paracrine growth factor signaling from stem cells to the trophectoderm
Alias Symbols: OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4
Protein Interaction Partner: ETS2, CETN2, XPC, RAD23B, UBC, ITCH, FOXD3, WWP2, PSMD10, NCL, PURA, PPIA, POU5F1, YBX1, KRT19, KRT14, KRT10, KRT9, KRT2, KRT1, HNRNPK, HMGB1, HIST1H1T, GAPDH, FKBP7, EHMT2, AGR2, TUBB4A, EIF5B, TUBA1A, NR2F2, TRA2B, SRSF3, RPS25, RPS23, RPS19, RPL31, RPL
Protein Size: 360 - Molecular Weight
- 39 kDa
- Gene ID
- 5460
- NCBI Accession
- NM_002701, NP_002692
- UniProt
- Q01860
- Pathways
- Stem Cell Maintenance
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