TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (N-Term) antibody

Details for Product No. ABIN2792680, Supplier: Log in to see
Antigen
  • CG17358
  • Dmel\\CG17358
  • T2DA_DROME
  • TAF
  • TAF12
  • TAF30a
  • TAF30alpha
  • TAF30alpha/28
  • TAF30alpha/28/22
  • TAFII30alpha/22
  • TAF[II]30alpha
  • TAF[[II]]
  • TAF[[II]]230
  • TAF[[II]]30alpha
  • TAF[[II]]30alpha/28 TAF[[II]]22
  • TAF[[II]]30alpha/28/22
  • TFIID
  • TFIID 22
  • TFIID 28
  • TFIID-p28
  • Taf22
  • Taf28
  • Taf30alpha
  • Taf32
  • Taf[[II]]30alpha
  • d28/22
  • dTAF12
  • dTAF22
  • dTAF28
  • dTAFII30
  • dTAFII30 alpha
  • dTAF[[II]]30
  • dTAF[[II]]30alpha
  • dTAF[[II]]30alpha/22
  • dmTAF12
  • p22
  • p28
  • 20kDa
  • 2810422D08Rik
  • AW557038
  • Taf2J
  • TAF2J
  • TAFII20
  • taf12-a
  • TBP-associated factor 12
  • TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor
  • TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa
  • Taf12
  • TAF12
  • taf12
Epitope
N-Term
11
3
2
2
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
46
27
25
3
3
3
3
2
2
2
2
2
2
1
1
Host
Rabbit
34
12
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
27
20
13
5
5
4
1
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human TAF12
Sequence MNQFGPSALI NLSNFSSIKP EPASTPPQGS MANSTAVVKI PGTPGAGGRL
Cross-Reactivity Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 85%, Rabbit: 100%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against TAF12. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name TAF12 (TAF12 Antibody Abstract)
Background Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I.Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: TAF2J, TAFII20
Protein Interaction Partner: PRKAR2B, SUMO1, UBE2I, TAF4B, KAT2B, HIST3H3, SUPT3H, TAF4, ERCC1, TAF10, ELAVL1, CPSF1, TAF3, TBP, TAF1A, TAF1C, ATF7, ATXN7, NFYC, NFYB, NFYA, TAF6, TAF8, TAF9, TAF11, TAF5, TAF15, TAF7, TAF2, TAF1, MYC,
Protein Size: 161
Molecular Weight 18 kDa
Gene ID 6883
NCBI Accession NM_005644, NP_005635
UniProt Q16514
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes WB Suggested Anti-TAF12 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: Transfected 293T.
Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 161 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (N-Term) antibody (ABIN2792680) WB Suggested Anti-TAF12 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Chromatin Immunoprecipitation (ChIP) image for anti-TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (N-Term) antibody (ABIN2792680) Quiescent human colon carcinoma HCT116 cultures were treated with 10 % FBS for three ...
Background publications Denissov, van Driel, Voit, Hekkelman, Hulsen, Hernandez, Grummt, Wehrens, Stunnenberg: "Identification of novel functional TBP-binding sites and general factor repertoires." in: The EMBO journal, Vol. 26, Issue 4, pp. 944-54, 2007 (PubMed).

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