NFATC3 antibody (N-Term)

Catalog No. ABIN2792685

Product Details anti-NFATC3 Antibody

Target: NFATC3 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 3 (NFATC3))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFATC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: AVFPFQYCVE TDIPLKTRKT SEDQAAILPG KLELCSDDQG SLSPARETSI

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against NFATC3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NFATC3

Application Details

Application Notes: WB Suggested Anti-NFATC3 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: Hela cell lysate.
Immunohistochemistry with Thymus tissue at an antibody concentration of 5 µg/mL using anti-NFATC3 antibody (ABIN504679).
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1068 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFATC3

Target: NFATC3 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 3 (NFATC3))

Alternative Name: NFAT4 / NFATC3 (NFATC3 Products)

Synonyms: NFATC3 antibody, NFATx antibody, nf-at antibody, nfat4 antibody, nfatx antibody, C80703 antibody, D8Ertd281e antibody, NFAT4 antibody, NFATX antibody, nuclear factor of activated T-cells 3 antibody, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 antibody, nuclear factor of activated T-cells 3 S homeolog antibody, NFATC3 antibody, nfatc3 antibody, Nfatc3 antibody, nfatc3.S antibody

Background: NFATC3 is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. NFATC3 plays a role in the regulation of gene expression in T cells and immature thymocytes.The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Four transcript variants encoding distinct isoforms have been identified for this gene.
Alias Symbols: NFAT4, NFATX
Protein Interaction Partner: MAPK9, MAPK8, CDK6, NCOA3, TTF1, CSNK1A1, FOS,
Protein Size: 1068

Molecular Weight: 115 kDa

Gene ID: 4775

NCBI Accession: NM_004555, NP_004546

UniProt: B5B2S0

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Chromatin Binding