» Antibodies » anti-IL-13 antibody (Biotin)

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IL-13 antibody (Biotin)

Antigen	IL-13
Clonality	Polyclonal
Host	Alternatives Rabbit Rabbit (27), Rat (5), Mouse (3)
Reactivity	Alternatives Human Human (27), Mouse (Murine) (20), Rat (Rattus) (17), Cow (Bovine) (16), Pig (Porcine) (16), Dog (Canine) (15), Sheep (Ovine) (15), Rabbit (2)
Conjugate	Alternatives Biotin Biotin (3), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
Application	Alternatives ELISA Flow Cytometry (FACS) (20), Immunofluorescence (IF) (18), ELISA (15), Western Blotting (WB) (11), Blocking Antibody (Inhibition) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Neutralization (Neut) (1)

Catalog no.	ABIN279997
Quantity	50 ug
Price	Product not available in this region.
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Additional Information
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Isotype	IgG
Specificity	The polyclonal antibody reacts with human interleukin-13 (IL-13). IL-13, also known as NC30, is a 12.5 kDa protein secreted by activated T helper cells, CD8+ T cells, and NK cells. The biological activities of IL-13 include suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression and suppression of inflammatory cytokine expression., Human

Application Details
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Application Notes	The biotinylated anti-human IL-13 polyclonal antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human IL-13 in combination with the purified PVM13-1 (ABIN281656) antibody for capture and recombinant human IL-13 (14-8139) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant cytokine standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate. The biotinylated anti-human IL-13 polyclonal antibody has been reported for use in ELISA and ELISPOT.
Buffer	Phosphate buffer pH 7.2,150 mM NaCl, 0.09% NaN3
Storage	Store at 4 °C.DO NOT FREEZE.
Research Area	Cytokines, Immunology, Virology, Inflammation, Cancer
Restrictions	For Research Use only

Images
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Human PBMCs (300,000/well) in 100 ul complete RPMI medium were treated without (left) or with (right) Candida (100ug/mL) for 24 hrs in an IL-13 ELISPOT assay plate (pre-coated with functional grade PVM13-1 antibody).

anti-IL-13 antibody (Biotin) (Image 2)

Alternatives
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Alternatives for antigen "IL-13", type "Antibodies"

Hosts	Rabbit (27), Rat (5), Mouse (3)
Reactivities	Human (27), Mouse (Murine) (20), Rat (Rattus) (17), Cow (Bovine) (16), Pig (Porcine) (16), Dog (Canine) (15), Sheep (Ovine) (15), Rabbit (2)
Applications	Flow Cytometry (FACS) (20), Immunofluorescence (IF) (18), ELISA (15), Western Blotting (WB) (11), Blocking Antibody (Inhibition) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Neutralization (Neut) (1)
Conjugates	Biotin (3), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1)