BUB1B antibody

Catalog No. ABIN1944865

Product Details anti-BUB1B Antibody

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1B antibody is un-conjugated

Application: Western Blotting (WB)

Isotype: IgG

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C,-20 °C

References for anti-BUB1B antibody (ABIN1944865)

Davenport, Fernandes, Harris, Neale, Goorha: "The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner." in: Genomics, Vol. 55, Issue 1, pp. 113-7, (1999) (PubMed).

Donadelli, Benatti, Remuzzi, Morigi, Gullans, Benigni, Remuzzi, Noris: "Identification of a novel gene--SSK1--in human endothelial cells exposed to shear stress." in: Biochemical and biophysical research communications, Vol. 246, Issue 3, pp. 881-7, (1998) (PubMed).

Taylor, Ha, McKeon: "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase." in: The Journal of cell biology, Vol. 142, Issue 1, pp. 1-11, (1998) (PubMed).

Chan, Schaar, Yen: "Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1." in: The Journal of cell biology, Vol. 143, Issue 1, pp. 49-63, (1998) (PubMed).

Cahill, Lengauer, Yu, Riggins, Willson, Markowitz, Kinzler, Vogelstein: "Mutations of mitotic checkpoint genes in human cancers." in: Nature, Vol. 392, Issue 6673, pp. 300-3, (1998) (PubMed).

Target Details for BUB1B

Target: BUB1B (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B))

Alternative Name: BUB1B (BUB1B Products)

Synonyms: BUB1beta antibody, BUBR1 antibody, Bub1A antibody, MAD3L antibody, MVA1 antibody, SSK1 antibody, hBUBR1 antibody, AU045529 antibody, bub1b antibody, BUB1 antibody, BUB1-like antibody, BubR1 antibody, BubRI antibody, CG14030 antibody, CG14030/Bub1 antibody, Dmel\\CG14030 antibody, bub1 antibody, BUB1 mitotic checkpoint serine/threonine kinase B antibody, BUB1B, mitotic checkpoint serine/threonine kinase antibody, BUB1B, mitotic checkpoint serine/threonine kinase L homeolog antibody, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta antibody, Bub1 kinase antibody, BUB1B antibody, Bub1b antibody, bub1b.L antibody, bub1b antibody, Bub1 antibody

Background: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

Molecular Weight: 119545 Da

Gene ID: 701

UniProt: O60566