LATS1 antibody (pThr1079) (Alexa Fluor 594)

Catalog No. ABIN2803713

Product Details anti-LATS1 Antibody (Alexa Fluor 594)

Target: LATS1 (LATS, Large Tumor Suppressor, Homolog 1 (Drosophila) (LATS1))

Binding Specificity: pThr1079

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LATS1 antibody is conjugated to Alexa Fluor 594

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Specificity: This sequence will cross react with LATS2 if phosphorylated at Thr1041

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human LATS1 around the phosphorylation site of Thr1079

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for LATS1

Target: LATS1 (LATS, Large Tumor Suppressor, Homolog 1 (Drosophila) (LATS1))

Alternative Name: LATS1 (LATS1 Products)

Synonyms: WARTS antibody, wts antibody, AW208599 antibody, Lats antibody, si:dkey-181i3.5 antibody, wu:fc19a11 antibody, zgc:123219 antibody, lats1 antibody, RGD1564085 antibody, warts antibody, large tumor suppressor kinase 1 antibody, large tumor suppressor antibody, large tumor suppressor kinase 1 S homeolog antibody, LATS1 antibody, Lats1 antibody, lats1 antibody, lats1.S antibody

Background:

Synonyms: wts, WARTS, Serine/threonine-protein kinase LATS1, Large tumor suppressor homolog 1, WARTS protein kinase, h-warts, LATS1

Background: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.

Gene ID: 9113

UniProt: O95835

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location