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Podocalyxin-Like (PODXL) antibody

Details for Product No. ABIN281752, Supplier: Log in to see
Antigen
  • AW121214
  • cPCLP1
  • fc18d02
  • Gp135
  • Gp200
  • Ly102
  • MEP21
  • PC
  • PCLP
  • PCLP-1
  • Pclp1
  • PCLP1
  • podocalyxin
  • Podxl
  • PODXL
  • podxl
  • wu:fc18d02
Reactivity
Human
176
35
29
11
3
2
1
1
1
1
Host
Mouse
89
83
15
8
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
14
12
11
8
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
133
66
65
37
34
29
26
24
14
4
3
2
2
1
Supplier
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Clone TRA-1-60
Isotype IgM
Specificity The TRA160 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA160 antibody is lost.
Characteristics The TRA-1-60 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.
Purification Purified
Alternative Name PODXL (PODXL Antibody Abstract)
Background Like other stem cell specific markers, the epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation. Contrary to early reports that the TRA-1-60 epitope can be destroyed by neuraminidase digestion, new strudies have shown that TRA-1-60 recognizes a neurominidase resistent antigen. The TRA-1-60 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.
Research Area Cardiovascular, Hematopoietic Progenitors, Extracellular Matrix
Application Notes Applications reported: This TRA-1-60 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting, and immunofluorescence.

Applications tested: This TRA-1-60 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test. This antibody has also been tested by immunoblotting of 2102Ep cell extracts, yielding a band of approximately 200-250 kDa (reducing and non-reducing conditions). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer aqueous buffer, 0.09 % sodium azide, contains carrier protein/stabilizer if necessary
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C
Supplier Images
 image for anti-Podocalyxin-Like (PODXL) antibody (ABIN281752) Staining of 2102Ep human embryonal carcinoma cells with 0.25 µg of Purified Mouse IgM...
Background publications Schopperle, DeWolf: "The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma." in: Stem cells (Dayton, Ohio), Vol. 25, Issue 3, pp. 723-30, 2007

Chin, Fong, Goh, Philp, Oh, Choo: "Identification of proteins from feeder conditioned medium that support human embryonic stem cells." in: Journal of biotechnology, Vol. 130, Issue 3, pp. 320-8, 2007

Badcock, Pigott, Goepel, Andrews: "The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan." in: Cancer research, Vol. 59, Issue 18, pp. 4715-9, 1999

Andrews, Banting, Damjanov, Arnaud, Avner: "Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells." in: Hybridoma, Vol. 3, Issue 4, pp. 347-61, 1985